Mouse Anti-CKB Recombinant Antibody (VS3-CJ615)
CAT#: VS3-CJ615
This product is a mouse antibody that recognizes human, and mouse CKB (Center).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1254/1472_C1_6_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1254/17_H1_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1254/18_H1_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1254/4140_B_9_5.jpg
Normal Tissue
Figure 5 Stomach
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1254/4140_A_1_1.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47313/104796_A_8_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CACO-2, HEK93, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166165-CKB
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Specifications
- Immunogen
- Purified His-tagged CKB protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse CKB (Center)
- Species Reactivity
- Human, Mouse
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000).
Target
- Alternative Names
- BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
- Gene ID
- 1152
- UniProt ID
- P12277
- Sequence Similarities
- Belongs to the ATP:guanido phosphotransferase family.
- Cellular Localization
- Cytoplasm, Mitochondrion
- Protein Refseq
- NP_001814.2
- Function
- Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255).
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity).
During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "CKB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1034 | Mouse Anti-CKB Recombinant Antibody (clone YC023); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0044MZ | Recombinant Mouse Anti-Human Creatine Kinase BB Antibody (clone A027-11288) | ELISA | Mouse antibody |
| MOB-0398CT | Recombinant Mouse anti-Human CKB Monoclonal antibody (4B7) | WB | |
| MRO-0387-CN | Rabbit Anti-CKB Recombinant Antibody (clone JB78-34) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX240 | Mouse Anti-CKB Recombinant Antibody (clone 4F3-H1-E8) | WB | Mouse IgG1 |
| VS3-QX241 | Mouse Anti-CKB Recombinant Antibody (clone 893CT29.1.1) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0708 | Hi-Affi™ Rabbit Anti-CKB Recombinant Antibody (clone DS708AB) | WB, IHC-P, FC, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1505 | AbPlus™ Anti-CKB Magnetic Beads (VS-0724-YC1505) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY464 | Anti-CKB Immunohistochemistry Kit | IHC | |
| VS-0525-XY1473 | Anti-Mouse CKB Immunohistochemistry Kit | IHC | |
| VS-0525-XY1472 | Anti-Human CKB Immunohistochemistry Kit | IHC |
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