Rabbit Anti-CLU Recombinant Antibody (clone R93-8K-4) (CAT#: VS3-FY2184)
This is a rabbit recombinant monoclonal antibody that is specificity for mouse CLU. It cross-reacts with rat. The R93-8K-4 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunoprecipitation (dilution 1/50).
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75983/1919_G12_2_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75983/1919_G12_2_selected.jpg
Figure 2 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75983/2064_D2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A549 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75983/2064_D2_1_blue_red_green.jpg
Figure 3 Cerebral cortex
(Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_B_7_5.jpg
(Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_B_7_5.jpg
Figure 4 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_A_6_6.jpg
Figure 5 Epididymis
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_A_3_6.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_A_3_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (HeLa, SK-BR-3, T-47d, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120885-CLU
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120885-CLU
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse and Rat CLU
- Species Reactivity
- Mouse, Rat
- Clone
- R93-8K-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 52 kDa, while the observed molecular weight stands at 40kDa.
- Related Disease
- Scrapie and Anaplastic Large Cell Lymphoma
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunoprecipitation: 1/50
Target
- Alternative Names
- Clusterin; Testosterone-Repressed Prostate Message 2; Apolipoprotein J; Complement-Associated Protein SP-40,40; Complement Cytolysis Inhibitor; Complement Lysis Inhibitor; Sulfated Glycoprotein 2; Ku70-Binding Protein 1; NA1/NA2; TRPM-2; APO-J; APOJ; KUB1
- Gene ID
- 1191
- UniProt ID
- P10909
- Sequence Similarities
- Belongs to the clusterin family.
- Cellular Localization
- Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Microsome
- Post Translation Modifications
- Proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen (PubMed:2387851).
Proteolytic cleavage is not necessary for its chaperone activity (PubMed:25402950).
All non-secreted forms are not proteolytically cleaved (PubMed:24073260).
Chaperone activity of uncleaved forms is dependent on a non-reducing environment.
- Protein Refseq
- NP_001822.3
- Function
- Functions as extracellular chaperone that prevents aggregation of non native proteins.
Prevents stress-induced aggregation of blood plasma proteins.
Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CLU"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-690-VHH | Recombinant Anti-Human CLU VHH Single Domain Antibody | ICC, FC, WB, FUNC | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2085z | Mouse Anti-CLU Recombinant Antibody (clone 25A5) | WB, IHC | Mouse IgG1 |
| MOB-0455MZ | Recombinant Mouse Anti-Rat CLU Antibody (clone 0.U.20) | WB | Mouse antibody |
| MOB-298YJL | Rabbit Anti-CLU Recombinant Antibody (clone CBC281) | ELISA | Rabbit IgG |
| ZG-0619J | Rabbit Anti-CLU Recombinant Antibody (clone 2A1) | ELISA, IHC | Rabbit IgG |
| VS-0723-WK113 | Human Anti-CLU Recombinant Antibody (VS-0723-WK113) | FC | Chimeric (rabbit/human) IgG1 |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-178CL | Human Anti-CLU Recombinant Antibody (TAB-178CL) | FuncS, Inhib | Human IgG2 |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533LC | Mouse Anti-CLU Recombinant Antibody (TAB-533LC) | WB | Mouse IgG |
| TAB-533LC-S(P) | Mouse Anti-CLU Recombinant Antibody; scFv Fragment (TAB-533LC-S(P)) | WB | Mouse scFv |
| TAB-533LC-F(E) | Mouse Anti-CLU Recombinant Antibody; Fab Fragment (TAB-533LC-F(E)) | WB | Mouse Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0735 | Hi-Affi™ Rabbit Anti-CLU Recombinant Antibody (clone DS735AB) | IHC-P, WB | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0203-YC-S(P) | Mouse Anti-CLU Recombinant Antibody; scFv Fragment (HPAB-0203-YC-S(P)) | ELISA, FC, RIA | Mouse scFv |
| HPAB-0204-YC-S(P) | Human Anti-CLU Recombinant Antibody; scFv Fragment (HPAB-0204-YC-S(P)) | ELISA, FC, RIA | Humanized scFv |
| HPAB-0440LY-S(P) | Human Anti-CLU Recombinant Antibody; scFv Fragment (HPAB-0440LY-S(P)) | ELISA, RIA, FC | Humanized scfv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0203-YC-F(E) | Mouse Anti-CLU Recombinant Antibody; Fab Fragment (HPAB-0203-YC-F(E)) | ELISA, FC, RIA | Mouse Fab |
| HPAB-0204-YC-F(E) | Human Anti-CLU Recombinant Antibody; Fab Fragment (HPAB-0204-YC-F(E)) | ELISA, FC, RIA | Humanized Fab |
| HPAB-0440LY-F(E) | Human Anti-CLU Recombinant Antibody; Fab Fragment (HPAB-0440LY-F(E)) | ELISA, RIA, FC | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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