Mouse Anti-CLU Recombinant Antibody (clone SAIC-43B-8)
CAT#: FAMAB-1093CQ
This product is a recombinant mouse antibody that recognizes CLU. The antibody was purified by affinity chromatography. It can be used in Immuno-MRM, WB, ELISA.
Published Data
Gene Expression
ELISA
Figure 1 ELISA data from screening recombinant monoclonal antibodies using recombinant proteins or overexpressed lysates.
ELISA data were reduced using Prism software to calculate the 50% binding point (B50%). The B50% values should not be interpreted as absolute numbers that define mAb performance (i.e., not analogously to affinity constants); instead, the B50% values can be used as relative values to compare the performances of the mAb within this group of mAbs.
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.
WB
Figure 2 Images of Western blots from screening the recombinant and hybridoma monoclonal antibodies (mAbs) using recombinant proteins, overexpressed lysates, and 6 breast cancer NCI60 cell line lysates.
The purified mAbs were run as controls alongside the recombinant proteins or the overexpressed lysate-proteins. Because the mAbs' protein targets (except for TSHB and CGB) were expected to be detected in breast cancer cell lines. For the Western blots using the NCI60 breast cancer cell lines.
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75983/1919_G12_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75983/2064_D2_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_B_7_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_A_6_6.jpg
Normal Tissue
Figure 5 Epididymis
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/572/1519_A_3_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HeLa, SK-BR-3, T-47d, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120885-CLU
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Specifications
- Immunogen
- Peptide ASSIIDELFQDR conjugated to KLH
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CLU
- Species Reactivity
- Human
- Clone
- SAIC-43B-8
- Applications
- Immuno-MRM, WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Clusterin; Testosterone-Repressed Prostate Message 2; Apolipoprotein J; Complement-Associated Protein SP-40,40; Complement Cytolysis Inhibitor; Complement Lysis Inhibitor; Sulfated Glycoprotein 2; Ku70-Binding Protein 1; NA1/NA2; TRPM-2; APO-J; APOJ; KUB1
- Gene ID
- 1191
- UniProt ID
- P10909
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone SAIC-43B-8"
- CAT
- Product Name
See other products for "CLU"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-690-VHH | Recombinant Anti-Human CLU VHH Single Domain Antibody | ICC, FC, WB, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-178CL | Human Anti-CLU Recombinant Antibody (TAB-178CL) | FuncS, Inhib | Human IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533LC | Mouse Anti-CLU Recombinant Antibody (TAB-533LC) | WB | Mouse IgG |
| TAB-533LC-S(P) | Mouse Anti-CLU Recombinant Antibody; scFv Fragment (TAB-533LC-S(P)) | WB | Mouse scFv |
| TAB-533LC-F(E) | Mouse Anti-CLU Recombinant Antibody; Fab Fragment (TAB-533LC-F(E)) | WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0735 | Hi-Affi™ Rabbit Anti-CLU Recombinant Antibody (clone DS735AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0203-YC | Mouse Anti-CLU Recombinant Antibody (HPAB-0203-YC) | ELISA, FC, RIA | Mouse IgG |
| HPAB-0204-YC | Human Anti-CLU Recombinant Antibody (HPAB-0204-YC) | ELISA, FC, RIA | Humanized IgG |
| HPAB-0440LY | Human Anti-CLU Recombinant Antibody (HPAB-0440LY) | ELISA, RIA, FC | Humanized IgG2 |
| VS-0723-WK113 | Human Anti-CLU Recombinant Antibody (VS-0723-WK113) | FC | Chimeric (rabbit/human) IgG1 |
| VS-0923-FY41 | Recombinant Mouse Anti-CLU Antibody (VS-0923-FY41) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0203-YC-S(P) | Mouse Anti-CLU Recombinant Antibody; scFv Fragment (HPAB-0203-YC-S(P)) | ELISA, FC, RIA | Mouse scFv |
| HPAB-0204-YC-S(P) | Human Anti-CLU Recombinant Antibody; scFv Fragment (HPAB-0204-YC-S(P)) | ELISA, FC, RIA | Humanized scFv |
| HPAB-0440LY-S(P) | Human Anti-CLU Recombinant Antibody; scFv Fragment (HPAB-0440LY-S(P)) | ELISA, RIA, FC | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0203-YC-F(E) | Mouse Anti-CLU Recombinant Antibody; Fab Fragment (HPAB-0203-YC-F(E)) | ELISA, FC, RIA | Mouse Fab |
| HPAB-0204-YC-F(E) | Human Anti-CLU Recombinant Antibody; Fab Fragment (HPAB-0204-YC-F(E)) | ELISA, FC, RIA | Humanized Fab |
| HPAB-0440LY-F(E) | Human Anti-CLU Recombinant Antibody; Fab Fragment (HPAB-0440LY-F(E)) | ELISA, RIA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1535 | Anti-CLU Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC66 | Recombinant Anti-CLU Eliminating Antibody, pH-Sensitive (VS-0625-YC66) | Antigen-Sweeping In Vivo. | Mouse IgG |
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