Mouse Anti-COL1A1 Recombinant Antibody (clone 2D10) (CAT#: ZG-0168J)
This product is a mouse antibody that recognizes COL1A1.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1934_F8_3_cr5d1ca7aa8d4b0_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1934_F8_3_cr5d1ca7aa8d4b0_selected.jpg
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows extracellular positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/ihc_selected.jpg
(Immunohistochemical staining of human colon shows extracellular positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/ihc_selected.jpg
Figure 3 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1936_A7_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1936_A7_4_blue_red_green.jpg
Figure 4 Cerebral cortex
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_7_5.jpg
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_7_5.jpg
Figure 5 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_8_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_8_5.jpg
Figure 6 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25% Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_5_6.jpg
(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25% Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_5_6.jpg
Figure 7 Skin
(Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_9_1.jpg
(Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_9_1.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC TERT1, BJ, fHDF/TERT166, HSkMC, U-138 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108821-COL1A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108821-COL1A1
Specifications
- Immunogen
- Synthetic Peptide of Collagen I
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human COL1A1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 2D10
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
- Alternative Names
- OI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; collagen alpha-1(I) chain; alpha-1 type I collagen; alpha1(I) procollagen; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain
- Gene ID
- 1277
- UniProt ID
- P02452
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-706-VHH | Recombinant Anti-Human COL1A1 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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