Rabbit Anti-COPS8 Recombinant Antibody (clone R05-6D7)
CAT#: VS3-FY1610
The rabbit recombinant monoclonal antibody is specificity for human COPS8. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Tested Data
Gene Expression
ICC
Figure 1 Anti-COPS8 Antibody (VS3-FY1610) in ICC.
Immunocytochemistry staining of COPS8 (green) in Hela using the anti-COPS8 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
IHC
Figure 2 Anti-COPS8 Antibody (VS3-FY1610) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer tissue using the anti-COPS8 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36485/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36485/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36485/73737_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36485/73737_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (hTERT-HME1, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198612-COPS8
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Specifications
- Immunogen
- Synthetic peptide of human COPS8
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat COPS8
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-6D7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 23 kDa, while the observed molecular weight stands at 23 kDa.
- Related Disease
- Xeroderma Pigmentosum
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- COP9 Signalosome Subunit 8; JAB1-Containing Signalosome Subunit 8; Signalosome Subunit 8; COP9 Homolog; HCOP9; CSN8
- Gene ID
- 10920
- UniProt ID
- Q99627
- Sequence Similarities
- Belongs to the CSN8 family.
- Cellular Localization
- Cytoplasm, Nucleus, Signalosome
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_006701.1; NP_937832.1
- Function
- Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "COPS8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1694MZ | Recombinant Mouse Anti-Human COPS8 Antibody | ELISA, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0766 | Hi-Affi™ Rabbit Anti-COPS8 Recombinant Antibody (clone DS766AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1615 | Anti-COPS8 Immunohistochemistry Kit | IHC |
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