Rabbit Anti-CPSF3 Recombinant Antibody (clone R98-6D-6) (CAT#: VS3-FY2159)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat CPSF3

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R98-6D-6

• Applications
• Western Blot, Immunofluorescence, Flow Cytometry

• Conjugate
• Unconjugated

• MW
• The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 77kDa.

• Related Disease
• Neurodevelopmental Disorder With Microcephaly, Hypotonia, Nystagmus, And Seizures and Neurodevelopmental Disorder With Hypotonia, Microcephaly, And Seizures

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4), 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200
  Flow Cytometry: 1/50

• Alternative Names
• Cleavage And Polyadenylation Specific Factor 3; Cleavage And Polyadenylation Specific Factor 3, 73kDa; MRNA 3-End-Processing Endonuclease CPSF-73; CPSF73; Cleavage And Polyadenylation Specificity Factor 73 KDa Subunit; Cleavage And Polyadenylation Specific Factor 3, 73kD Subunit

• Gene ID
• 51692

• UniProt ID
• Q9UKF6

• Sequence Similarities
• Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. (Q9UKF6-CPSF3_HUMAN)
  Ubiquitination at Lys295, Lys306, Lys428, and Lys487 (NX_Q9UKF6 [NX_Q9UKF6-1])
  Modification sites at PhosphoSitePlus (Q9UKF6)
  Glycosylation from GlyGen (Q9UKF6) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_047300701.1; XP_054198526.1; NP_001308762.1; NP_001308763.1; NP_001308764.1

• Function
• Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease.
  Also involved in the histone 3'-end pre-mRNA processing.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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