Rabbit Anti-CPSF3 Recombinant Antibody (clone R98-6D-6)
CAT#: VS3-FY2159
This is a rabbit recombinant monoclonal antibody that is involved in the recognition of human CPSF3 protein, a calculated weight of 77 kDa. It cross-reacts with mouse, rat. The R98-6D-6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), flow cytometry (dilution 1/50).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34657/74423_A_4_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34657/74423_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34657/74423_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34657/74423_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34657/74423_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34657/74423_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34657/74423_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119203-CPSF3
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CPSF3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R98-6D-6
- Applications
- Western Blot, Immunofluorescence, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 77kDa.
- Related Disease
- Neurodevelopmental Disorder With Microcephaly, Hypotonia, Nystagmus, And Seizures and Neurodevelopmental Disorder With Hypotonia, Microcephaly, And Seizures
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Flow Cytometry: 1/50
Target
- Alternative Names
- Cleavage And Polyadenylation Specific Factor 3; Cleavage And Polyadenylation Specific Factor 3, 73kDa; MRNA 3-End-Processing Endonuclease CPSF-73; CPSF73; Cleavage And Polyadenylation Specificity Factor 73 KDa Subunit; Cleavage And Polyadenylation Specific Factor 3, 73kD Subunit
- Gene ID
- 51692
- UniProt ID
- Q9UKF6
- Sequence Similarities
- Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. (Q9UKF6-CPSF3_HUMAN)
Ubiquitination at Lys295, Lys306, Lys428, and Lys487 (NX_Q9UKF6 [NX_Q9UKF6-1])
Modification sites at PhosphoSitePlus (Q9UKF6)
Glycosylation from GlyGen (Q9UKF6) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047300701.1; XP_054198526.1; NP_001308762.1; NP_001308763.1; NP_001308764.1
- Function
- Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease.
Also involved in the histone 3'-end pre-mRNA processing.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CPSF3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0731MZ | Recombinant Mouse Anti-Human CPSF3 Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0787 | Hi-Affi™ Rabbit Anti-CPSF3 Recombinant Antibody (clone DS787AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY528 | Anti-CPSF3 Immunohistochemistry Kit | IHC |
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