This product is a recombinant antibody that recognizes CRTAM. The monoclonal antibody 17B2 reacts with the epitope of CRTAM.
Figure 1 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-1089-FY in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 2 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SEC-HPLC
The purity of HPAB-1089-FY was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in DB
Dot Blot analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag).
HPAB-1089-FY incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 4 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in WB
Western blot analysis of HPAB-1089-FY was performed by loading human CRTAM protein, His tag.
HPAB-1089-FY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
Figure 5 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in ELISA
ELISA analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag). Then blocked with BSA and incubated with HPAB-1089-FY. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2006MZ | Recombinant Mouse Anti-Human CRTAM Antibody (clone NN0305-32B0) | FC, WB | Mouse antibody |
VS-0723-WK71 | Human Anti-CRTAM Recombinant Antibody (VS-0723-WK71) | FC | Chimeric (rabbit/human) IgG1 |
VS-0723-WK81 | Human Anti-CRTAM Recombinant Antibody (VS-0723-WK81) | FC | Chimeric (rabbit/human) IgG1 |
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-1089-FY, RRID: AB_3111415)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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