Mouse Anti-CRTAM Recombinant Antibody (clone 17B2) (CAT#: HPAB-1089-FY)
This product is a recombinant antibody that recognizes CRTAM. The monoclonal antibody 17B2 reacts with the epitope of CRTAM.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
SDS-PAGE
Figure 1 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-1089-FY in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SEC-HPLC
The purity of HPAB-1089-FY was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in DB
Dot Blot analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag).
HPAB-1089-FY incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
WB
Figure 4 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in WB
Western blot analysis of HPAB-1089-FY was performed by loading human CRTAM protein, His tag.
HPAB-1089-FY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 5 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in ELISA
ELISA analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag). Then blocked with BSA and incubated with HPAB-1089-FY. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Mouse
- Derivation
- Chimeric (hamster/mouse)
- Type
- Mouse IgG
- Specificity
- Human CRTAM
- Species Reactivity
- Human
- Clone
- 17B2
- Applications
- WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cytotoxic And Regulatory T Cell Molecule; Class-I MHC-Restricted T-Cell-Associated Molecule; Cytotoxic And Regulatory T-Cell Molecule; Class I MHC Restricted T Cell Associated Molecule; CD355 Antigen; CD355
- Gene ID
- 56253
- UniProt ID
- O95727
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2006MZ | Recombinant Mouse Anti-Human CRTAM Antibody (clone NN0305-32B0) | FC, WB | Mouse antibody |
| VS-0723-WK71 | Human Anti-CRTAM Recombinant Antibody (VS-0723-WK71) | FC | Chimeric (rabbit/human) IgG1 |
| VS-0723-WK81 | Human Anti-CRTAM Recombinant Antibody (VS-0723-WK81) | FC | Chimeric (rabbit/human) IgG1 |
Customer Reviews and Q&As
Customer Review
Q&As
High Batch Consistency
We've ordered multiple batches of this antibody, and each batch has been consistent in quality and performance.
Good Value
Considering the high quality and performance, this antibody is very cost-effective. Great value for the price.
Perfect for Research
This is an ideal research antibody with proven reliability in our lab. It meets all our research needs effectively.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-1089-FY, RRID: AB_3111415)
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For Research Use Only. Not For Clinical Use.
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