Rabbit Anti-CSNK2A1 Recombinant Antibody (clone R05-7F8) (CAT#: VS3-FY1990)

• Immunogen
• Synthetic peptide of human CSNK2A1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat CSNK2A1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R05-7F8

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 45 kDa, while the observed molecular weight stands at 45 kDa.

• Related Disease
• Okur-Chung Neurodevelopmental Syndrome and Cardiomyopathy, Dilated, 1P

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Casein Kinase 2 Alpha 1; Casein Kinase 2, Alpha 1 Polypeptide; EC 2.7.11.1; CK2A1; Casein Kinase II Alpha 1 Polypeptide Pseudogene; Casein Kinase II Alpha 1 Subunit; Casein Kinase II Subunit Alpha; CK2 Catalytic Subunit Alpha; Protein Kinase CK2

• Gene ID
• 1457

• UniProt ID
• P68400

• Sequence Similarities
• Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylated at Thr-344, Thr-360, Ser-362 and Ser-370 by CDK1 in prophase and metaphase and dephosphorylated during anaphase.
  Phosphorylation does not directly affect casein kinase 2 activity, but may contribute to its regulation by forming binding sites for interacting proteins and/or targeting it to different compartments.
  (P68400-CSK21_HUMAN)
  Ubiquitination at Lys229 (NX_P68400 [NX_P68400-1])
  Modification sites at PhosphoSitePlus (P68400)
  Glycosylation from GlyGen (P68400) 3 sites, 1 O-linked glycan (3 sites)

• Protein Refseq
• NP_001349699.1; NP_001349700.1; NP_001886.1; NP_808227.1; NP_808228.1

• Function
• Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.
  Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection.
  May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response.
  During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage.
  Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody