SmartAb™ Recombinant Anti-CX3CL1 pH-dependent Antibody (VS-0825-YC88)
CAT#: VS-0825-YC88
The anti-CX3CL1 pH-dependent antibody (VS-0825-YC88) targets human CX3CL1 in a pH-dependent manner, with its affinity remaining high at neutral pH but weakening at a lower pH, such as within the acidic environment of endosomes. The anti-CX3CL1 acid-switched antibody was engineered by incorporating histidine residues into its variable regions. The introduction of histidine substitutions within its Complementarity-Determining Regions (CDRs) enables pH-responsive antigen binding, which leads to enhanced CX3CL1 clearance. The antibody operates in a cyclical manner, binding CX3CL1 in the extracellular fluid and releasing it in acidified endosomes. Human CX3CL1, also known as Fractalkine, is a chemokine that exists in both soluble and membrane-bound forms. It attracts T-cells, NK cells, and monocytes and is a key player in inflammation and immune cell trafficking in tissues.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26192/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26192/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40361/90576_A_8_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40361/90576_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40361/90576_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40361/90576_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40361/90576_A_5_6.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40361/90576_B_9_1.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (EFO-21, HDLM-2, Hep G2, RPTEC TERT1, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000006210-CX3CL1
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG
- Specificity
- Human CX3CL1
- Species Reactivity
- Human
- Applications
- Neut
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
- Alternative Names
- C-X3-C Motif Chemokine Ligand 1; Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member 1 (Fractalkine, Neurotactin); Chemokine (C-X3-C Motif) Ligand 1; CX3C Membrane-Anchored Chemokine; Small-Inducible Cytokine D1; C-X3-C Motif Chemokine 1; Neurotactin; SCYD1; NTT
- Gene ID
- 6376
- UniProt ID
- P78423
- Long Name
- C-X3-C Motif Chemokine Ligand 1
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2061MZ | Recombinant Mouse Anti-Human CX3CL1 Antibody (clone NN0308-9K34) | IHC-P, WB | Mouse antibody |
| HPAB-1500-FY | Human Anti-CX3CL1 Recombinant Antibody (HPAB-1500-FY) | Neut | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-582CQ | Rat Anti-Cx3cl1 Recombinant Antibody (clone 7F17) | Neut, WB | Rat IgG2 |
| NEUT-583CQ | Mouse Anti-CX3CL1 Recombinant Antibody (clone CBL828) | CyTOF®, ELISA, ICFC, Neut | Mouse IgG1 |
| NEUT-584CQ | Rat Anti-Cx3cl1 Recombinant Antibody (clone CBL858) | Neut, WB | Rat IgG2 |
| NEUT-585CQ | Rat Anti-Cx3cl1 Recombinant Antibody (clone CBL690) | ELISA, WB, Neut | Rat IgG2a |
| NEUT-586CQ | Rat Anti-Cx3cl1 Recombinant Antibody (clone RM0200-7F17) | WB, Neut | Rat IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0880 | Hi-Affi™ Rabbit Anti-CX3CL1 Recombinant Antibody (clone DS880AB) | ELISA, WB | Rabbit IgG |
| MOR-4123 | Hi-Affi™ Rabbit Anti-CX3CL1 Recombinant Antibody (clone SI194DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1500-FY-F(E) | Human Anti-CX3CL1 Recombinant Antibody; Fab Fragment (HPAB-1500-FY-F(E)) | Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1500-FY-S(P) | Human Anti-CX3CL1 Recombinant Antibody; scFv Fragment (HPAB-1500-FY-S(P)) | Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY590 | Anti-CX3CL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1809 | Anti-Human CX3CL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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