Rabbit Anti-CYP1A2 Recombinant Antibody (ZG-0631J)
CAT#: ZG-0631J
This product is a rabbit antibody that recognizes CYP1A2.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-CYP1A2 Antibody (ZG-0631J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, L02 whole cell lysate
All lanes: CYP1A2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
IHC
Figure 2 Rabbit Anti-CYP1A2 Antibody (ZG-0631J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Normal Tissue
Figure 1 High expression in liver.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16531/36095_A_9_4_val_selected.jpg
Normal Tissue
Figure 2 Low expression in colon.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16531/36095_A_7_3_val_selected.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16531/36095_A_7_4.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000140505-CYP1A2
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Specifications
- Immunogen
- A synthesized peptide derived from human Cytochrome P450
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CYP1A2
- Species Reactivity
- Human
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- CP12; CYPIA2; P3-450; P450(PA); cytochrome P450 1A2; P450 form 4; aryl hydrocarbon hydroxylase; cholesterol 25-hydroxylase; cytochrome P(3)450; cytochrome P450 4; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; xenobiotic monooxygenase
- Gene ID
- 1544
- UniProt ID
- P05177
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CYP1A2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1376z | Mouse Anti-CYP1A2 Recombinant Antibody (clone 12H7) | WB, ICC, IF, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0191CT | Recombinant Mouse anti-Human CYP1A2 Monoclonal antibody (EML757) | IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0900 | Hi-Affi™ Rabbit Anti-CYP1A2 Recombinant Antibody (clone DS900AB) | WB, FC, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0717F | Mouse Anti-CYP1A2 Recombinant Antibody (clone 3B8C1) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0819F | Mouse Anti-CYP1A2 Antibody (clone F16 P2 D8) | WB, ELISA, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY560 | Mouse Anti-CYP1A2 Recombinant Antibody (clone 499CT13.2.2) | WB | Mouse IgG3 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1851 | Anti-CYP1A2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1852 | Anti-Mouse CYP1A2 Immunohistochemistry Kit | IHC |
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