Mouse Anti-Cytochrome B Recombinant Antibody (clone NS5) (CAT#: FAMAB-0189JF)
Recombinant monoclonal antibody specially binds to human Flavocytochrome b. This mouse anti-cytochrome B antibody (NS5) can be a useful tool to study vascular physiology.
Specific Inquiry
WB
Figure 1 Western blots of R3179 IgG to cytochrome b preparations and CGD neutrophils.
Protein samples were subject to SDS-PAGE and then transferred to nitrocellulose as described.
Parkos, C. A., Allen, R. A., Cochrane, C. G., & Jesaitis, A. J. (1987). Purified cytochrome b from human granulocyte plasma membrane is comprised of two polypeptides with relative molecular weights of 91,000 and 22,000. The Journal of clinical investigation, 80(3), 732-742.
ELISA
Figure 2 Elution of cytochrome b from heparin ultrogel.
Eluate from WGA-4B containing 35.8 nmol of cytochrome b was pumped through a 5-ml column of heparin ultrogel as described.
Parkos, C. A., Allen, R. A., Cochrane, C. G., & Jesaitis, A. J. (1987). Purified cytochrome b from human granulocyte plasma membrane is comprised of two polypeptides with relative molecular weights of 91,000 and 22,000. The Journal of clinical investigation, 80(3), 732-742.
IHC
Figure 3 Immunostaining (20×)for BPDE-DNA adducts in hepatic tissue sections of HRN.
No staining was observed in control (untreated) animals.
Arlt, V. M., Poirier, M. C., Sykes, S. E., John, K., Moserova, M., Stiborova, M., ... & Phillips, D. H. (2012). Exposure to benzo [a] pyrene of hepatic cytochrome P450 reductase null (HRN) and P450 reductase conditional null (RCN) mice: detection of benzo [a] pyrene diol epoxide-DNA adducts by immunohistochemistry and 32P-postlabelling. Toxicology letters, 213(2), 160-166.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Cytochrome b
- Species Reactivity
- Human
- Clone
- NS5
- Applications
- WB, ELISA, IHC
- Related Disease
- Chronic granulomatous
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The cytochrome B antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0188JF | Mouse Anti-Cytochrome B Recombinant Antibody (clone 44.1) | WB, FC, IHC | Mouse IgG |
| FAMAB-0190JF | Mouse Anti-Cytochrome B Recombinant Antibody (clone NL7) | WB, ELISA, FC, IHC | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0188JF-F(E) | Mouse Anti-Cytochrome B Recombinant Antibody (clone 44.1); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0189JF-F(E) | Mouse Anti-Cytochrome B Recombinant Antibody (clone NS5); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0190JF-F(E) | Mouse Anti-Cytochrome B Recombinant Antibody (clone NL7); Fab Fragment | ELISA, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0188JF-S(P) | Mouse Anti-Cytochrome B Recombinant Antibody (clone 44.1); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0189JF-S(P) | Mouse Anti-Cytochrome B Recombinant Antibody (clone NS5); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0190JF-S(P) | Mouse Anti-Cytochrome B Recombinant Antibody (clone NL7); scFv Fragment | ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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