Mouse Anti-DAGLA Recombinant Antibody (clone 2506W50)
CAT#: VS-0625-FY20
This Mouse IgG1 kappa monoclonal antibody, clone 2506W50, effectively targets DAGLα in Human, Mouse, and Rat. Its immunogen was raised against amino acids 887-1042 of human DAGLα. With an observed antigen size of ~37 kDa (calculated 51.58 kDa), this antibody is validated for Western Blot, Immunoprecipitation, Immunofluorescence, and ELISA, making it a valuable tool for DAGLα research.
Specifications
- Immunogen
- Antibody raised against human DAGLα, targeting amino acids 887-1042 within a C-terminal cytoplasmic domain.
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 2506W50
- Applications
- Western Blot, Immunoprecipitation, Immunofluorescence, ELISA
- Related Disease
- Neuroocular Syndrome 2, Paroxysmal Type and Spinocerebellar Ataxia 20
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Diacylglycerol Lipase Alpha; Neural Stem Cell-Derived Dendrite Regulator; DGL-Alpha; C11orf11; NSDDR; Sn1-Specific Diacylglycerol Lipase Alpha; Chromosome 11 Open Reading Frame 11
- Gene ID
- 747
- UniProt ID
- Q9Y4D2
- Long Name
- Diacylglycerol Lipase Alpha
- Sequence Similarities
- Belongs to the AB hydrolase superfamily. Lipase family.
- Cellular Localization
- Cell membrane; Multi-pass membrane protein
- Post Translation Modifications
- Phosphorylated at Ser-782 and Ser-808 by CAMK2A; phosphorylation by CAMK2A inhibits diacylglycerol lipase activity.
- Protein Refseq
- XP_047283496.1; XP_047283497.1; XP_047283498.1; XP_047283499.1; XP_047283500.1
- Function
- Serine hydrolase that hydrolyzes arachidonic acid-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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