Mouse Anti-DCX Recombinant Antibody (clone 1/108) (CAT#: VS-0524-YC20)
This is a new mouse monoclonal antibody against native human doublecortin. The antibody binds the protein in ELISA but not in Western blot experiments. Clone 1/108 binds specifically the N-terminal domain of doublecortin and could be a unique research tool to distinguish the roles of individual DCX domains in doublecortin function. 1/108 did not interfere with microtubule binding of T-DCX. 1/108 recognizes the N-DCX loops 142-146, 174-177, and 213-216.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/1373_A2_4_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/1373_A2_4_selected.jpg
Figure 2 Colon
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_7_3.jpg
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_7_3.jpg
Figure 3 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_8_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_8_5.jpg
Figure 4 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_6_6.jpg
Figure 5 RNA cell line category: Group enriched (SCLC-21H, SH-SY5Y)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077279-DCX
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077279-DCX
Specifications
- Immunogen
- Native human doublecortin
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG2b
- Specificity
- Human doublecortin
- Species Reactivity
- Human
- Clone
- 1/108
- Applications
- ELISA
- Related Disease
- Lissencephaly, X-Linked, 1 and Band Heterotopia.
Product Property
- Purification
- Protein G purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Doublecortin; Lissencephalin-X; Doublecortex; Doublin; Lis-X; DBCN; LISX;
- Gene ID
- 1641
- UniProt ID
- O43602
- Long Name
- doublecortin
- Cellular Localization
- Cytoplasm; Cell projection, neuron projection
- Post Translation Modifications
- Phosphorylation (at Ser-265 and Ser-297) by MARK1, MARK2 and PKA regulates its ability to bind microtubules.
Ubiquitinated by MDM2, leading to its degradation by the proteasome.
- Protein Refseq
- NP_000546.2; NP_001182482.1; NP_001356299.1; NP_001356300.1; NP_001356301.1
- Function
- May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein.
May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration.
May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0594CT | Recombinant Mouse anti-Human DCX Monoclonal antibody (EML131) | ELISA, ICC/IF, WB | |
| MRO-0491-CN | Rabbit Anti-DCX Recombinant Antibody (clone JJ0959) | WB, FC | Rabbit IgG |
| PABS-0131 | Camelid Anti-DCX Recombinant Single Domain Antibody (Clone XA4551) | ELISA, FuncS | Camelid VHH |
| VS3-WK1824 | Recombinant Rabbit Anti-DCX Capture Antibody (clone R34-7M-3) | ELISA | Rabbit IgG |
| VS3-WK1825 | Recombinant Rabbit Anti-DCX Detection Antibody (clone R16-8A-1) | ELISA | Rabbit IgG |
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