Mouse Anti-DCX Recombinant Antibody (clone 1/108) (CAT#: VS-0524-YC20)
This is a new mouse monoclonal antibody against native human doublecortin. The antibody binds the protein in ELISA but not in Western blot experiments. Clone 1/108 binds specifically the N-terminal domain of doublecortin and could be a unique research tool to distinguish the roles of individual DCX domains in doublecortin function. 1/108 did not interfere with microtubule binding of T-DCX. 1/108 recognizes the N-DCX loops 142-146, 174-177, and 213-216.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/1373_A2_4_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/1373_A2_4_selected.jpg
Figure 2 Colon
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_7_3.jpg
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_7_3.jpg
Figure 3 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_8_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_8_5.jpg
Figure 4 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36121/166947_A_6_6.jpg
Figure 5 RNA cell line category: Group enriched (SCLC-21H, SH-SY5Y)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077279-DCX
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077279-DCX
Specifications
- Immunogen
- Native human doublecortin
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG2b
- Specificity
- Human doublecortin
- Species Reactivity
- Human
- Clone
- 1/108
- Applications
- ELISA
- Related Disease
- Lissencephaly, X-Linked, 1 and Band Heterotopia.
Product Property
- Purification
- Protein G purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Doublecortin; Lissencephalin-X; Doublecortex; Doublin; Lis-X; DBCN; LISX;
- Gene ID
- 1641
- UniProt ID
- O43602
- Long Name
- doublecortin
- Cellular Localization
- Cytoplasm; Cell projection, neuron projection
- Post Translation Modifications
- Phosphorylation (at Ser-265 and Ser-297) by MARK1, MARK2 and PKA regulates its ability to bind microtubules.
Ubiquitinated by MDM2, leading to its degradation by the proteasome.
- Protein Refseq
- NP_000546.2; NP_001182482.1; NP_001356299.1; NP_001356300.1; NP_001356301.1
- Function
- May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein.
May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration.
May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "DCX"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
Antibody Magnetic Beads
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0594CT | Recombinant Mouse anti-Human DCX Monoclonal antibody (EML131) | ELISA, ICC/IF, WB | |
| MRO-0491-CN | Rabbit Anti-DCX Recombinant Antibody (clone JJ0959) | WB, FC | Rabbit IgG |
| PABS-0131 | Camelid Anti-DCX Recombinant Single Domain Antibody (Clone XA4551) | ELISA, FuncS | Camelid VHH |
| VS3-WK1824 | Recombinant Rabbit Anti-DCX Capture Antibody (clone R34-7M-3) | ELISA | Rabbit IgG |
| VS3-WK1825 | Recombinant Rabbit Anti-DCX Detection Antibody (clone R16-8A-1) | ELISA | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-0524-YC20. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: ELISA, Inhib, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, WB, FC, IHC, IP
-1.jpg)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
