Mouse Anti-DDIT3 Recombinant Antibody (VS13-YC291) (CAT#: VS13-YC291)
The Mouse Anti-DDIT3 Recombinant Antibody is specific for human, mouse and rat DDIT3. DDIT3 encodes a transcription factor from the C/EBP family, acting as a dominant inhibitor influencing adipogenesis and apoptosis through stress-activated pathways. The anti-DDIT3 antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunofluorescence (1/50-1/200).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58416/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58416/if_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_B_7_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_7_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_4_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_9_8.jpg
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTERT-HME1, SK-MEL-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175197-DDIT3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175197-DDIT3
Specifications
- Immunogen
- Recombinant DDIT3 conjugated to KLH.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Rat DDIT3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.2) containing 50% Glycerol and 0.5% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- DNA Damage Inducible Transcript 3; C/EBP Zeta; Growth Arrest And DNA Damage-Inducible Protein GADD153; CCAAT/Enhancer-Binding Protein Homologous Protein; C/EBP-Homologous Protein 10; CHOP-10; GADD153; CHOP10;
- Gene ID
- 1649
- UniProt ID
- P35638
- Long Name
- DNA Damage Inducible Transcript 3
- Cellular Localization
- Nucleus; Cytoplasm
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3990z | Mouse Anti-DDIT3 Recombinant Antibody (clone 20G2) | WB, FC, ICC, IHC | Mouse IgG2a |
| MOB-2287CT | Recombinant Mouse anti-Human DDIT3 Monoclonal antibody (FQS4680) | WB, IP, ICC/IF, IHC-Fr, IHC-P | |
| VS3-FY381 | Recombinant Mouse Anti-DDIT3 Antibody (clone 1E1) | IHC | Mouse IgG |
| VS3-FY382 | Recombinant Mouse Anti-DDIT3 Antibody (clone 2B1) | WB, IHC | Mouse IgG |
| VS3-FY447 | Recombinant Rabbit Anti-DDIT3 Antibody (clone R02-6A3) | WB, IHC-P | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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