Provided is a human anti-dengue virus antibody (an anti-DENV antibody) that binds to a DENV envelope protein and is cross-reactive with DENV serotype 1, DENV serotype 2, DENV serotype 3, and DENV serotype 4.
Figure 1 Binding of m366.6 to DENV DIIIs from the four serotypes measured by Biacore.
The m366.6 was immobilized onto a CM5 chip, and the analytes consisted of serial dilution of DIII from DENV-1 (A), DENV-2 (B), DENV-3 (C), or DENV-4 (D). Binding kinetics was fitted using a 1:1 Langumir binding model by BIAevaluation 3.2 software.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Figure 2 eutralization and ADE effects of antibodies against different serotypes DENV.
DENV1-4 was mixed with 5-fold serial dilutions of mabs. Then neutralization activity was evaluated by plaque reduction assays in triplicates using BHK21 cells. Results were exhibited by percentage of plaques reduction and the independent neutralization experiments were performed in duplicate.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Figure 3 In vivo therapeutic efficacy of m366.6 against DENV1-4 infection.
Groups of one day old suckling mice were administrated with 100 μg of mAbs 4 hours after challenge with 200 PFU of DENV-1 (A), DENV-2 (B), DENV-3 (C), and DENV-4 (D), respectively. Unrelated antibody G12 was used in the negative controls. The number of animals for each group ranged from 6 to 9. Kaplan-Meier survival curves were analyzed by the log-rank test and compared to curves of the controls.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Figure 4 Binding of m366.6 to DENV DIII, ZIKV DIII, gp140 and PDL1 proteins measured by ELISA.
Binding of m366.6 to DENV DIII, ZIKV DIII, gp140 and PDL1 proteins measured by ELISA.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0154CQ | Recombinant Human Anti-DENV Antibody (MAb 1A5) | ELISA, Neut, WB, FuncS | Human IgG1 |
HPAB-0128-YJ | Human Anti-DENV Recombinant Antibody (HPAB-0128-YJ) | ELISA, Neut, FuncS | Humanized IgG |
HPAB-0129-YJ | Human Anti-DENV Recombinant Antibody (HPAB-0129-YJ) | ELISA, Neut, FuncS | Humanized IgG |
HPAB-M0048-YC | Recombinant Mouse Anti-DENV Antibody (A11) | ELISA, Neut | Mouse IgG |
HPAB-M0049-YC | Recombinant Mouse Anti-DENV Antibody (HPAB-M0049-YC) | ELISA, Neut | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0154CQ-S(P) | Recombinant Human Anti-DENV Antibody scFv Fragment (MAb 1A5) | ELISA, Neut | Human scFv |
HPAB-1281LY-S(P) | Mouse Anti-DENV Recombinant Antibody; scFv Fragment (HPAB-1281LY-S(P)) | Neut, IHC, ELISA | Mouse scFv |
HPAB-2089LY-S(P) | Mouse Anti-DENV Recombinant Antibody; scFv Fragment (HPAB-2089LY-S(P)) | ELISA, RIA, WB | Mouse scFv |
HPAB-AP058-YC-S(P) | Recombinant Human Anti-DENV Antibody scFv Fragment (m366) | ELISA, FC, Neut | |
HPAB-AP059-YC-S(P) | Recombinant Human Anti-DENV Antibody scFv Fragment (HPAB-AP059-YC-S(P)) | ELISA, FC, Neut |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0128-YJ-F(E) | Human Anti-DENV Recombinant Antibody; Fab Fragment (HPAB-0128-YJ-F(E)) | ELISA, Neut, FuncS | Humanized Fab |
HPAB-0129-YJ-F(E) | Human Anti-DENV Recombinant Antibody; Fab Fragment (HPAB-0129-YJ-F(E)) | ELISA, Neut, FuncS | Humanized Fab |
HPAB-AP058-YC-F(E) | Recombinant Human Anti-DENV Antibody Fab Fragment (m366) | ELISA, FC, Neut | |
HPAB-AP059-YC-F(E) | Recombinant Human Anti-DENV Antibody Fab Fragment (HPAB-AP059-YC-F(E)) | ELISA, FC, Neut | |
HPAB-AP060-YC-F(E) | Recombinant Human Anti-DENV Antibody Fab Fragment (m360) | ELISA, FC, Neut |
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