Recombinant Human Anti-DENV Antibody (m366.6)
CAT#: HPAB-AP059-YC
Provided is a human anti-dengue virus antibody (an anti-DENV antibody) that binds to a DENV envelope protein and is cross-reactive with DENV serotype 1, DENV serotype 2, DENV serotype 3, and DENV serotype 4.
Published Data
SPR
Figure 1 Binding of m366.6 to DENV DIIIs from the four serotypes measured by Biacore.
The m366.6 was immobilized onto a CM5 chip, and the analytes consisted of serial dilution of DIII from DENV-1 (A), DENV-2 (B), DENV-3 (C), or DENV-4 (D). Binding kinetics was fitted using a 1:1 Langumir binding model by BIAevaluation 3.2 software.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Neut
Figure 2 eutralization and ADE effects of antibodies against different serotypes DENV.
DENV1-4 was mixed with 5-fold serial dilutions of mabs. Then neutralization activity was evaluated by plaque reduction assays in triplicates using BHK21 cells. Results were exhibited by percentage of plaques reduction and the independent neutralization experiments were performed in duplicate.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
FuncS
Figure 3 In vivo therapeutic efficacy of m366.6 against DENV1-4 infection.
Groups of one day old suckling mice were administrated with 100 μg of mAbs 4 hours after challenge with 200 PFU of DENV-1 (A), DENV-2 (B), DENV-3 (C), and DENV-4 (D), respectively. Unrelated antibody G12 was used in the negative controls. The number of animals for each group ranged from 6 to 9. Kaplan-Meier survival curves were analyzed by the log-rank test and compared to curves of the controls.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
ELISA
Figure 4 Binding of m366.6 to DENV DIII, ZIKV DIII, gp140 and PDL1 proteins measured by ELISA.
Binding of m366.6 to DENV DIII, ZIKV DIII, gp140 and PDL1 proteins measured by ELISA.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
❮
❯
Specifications
- Host Species
- Human
- Derivation
- Human antibody library
- Specificity
- DENV
- Species Reactivity
- DENV
- Clone
- m366.6
- Applications
- SPR, Neut, ELISA, Funcs, FC
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Applications
- Application Notes
- The DENV antibody has been reported in applications of Surface Plasmon Resonance, Neutralization, Enzyme-linked Immunosorbent Assay, Functional Assay, Flow Cytometry.
For Surface Plasmon Resonance: Binding affinities of m360 scFv, m366 scFv, m360.6 scFv, and m366.6 scFv to the 4 DENV DIIIs were analyzed by surface plasmon resonance technology using a Biacore X100 instrument. The antibodies were covalently immobilized onto a sensor chip (CM5) using carbodiimide coupling chemistry. A control reference surface was prepared for nonspecific binding and refractive index changes. For analysis of the kinetics of interactions, varying concentrations of antigens were injected at flow rate of 30 μl/min using running buffer containing 10mM HEPES, 150 mM NaCl, 3 mM EDTA, and 0.05% Surfactant P-20 (pH 7.4). The association and dissociation phase data were fitted simultaneously to a 1:1 Langumir global model by using the nonlinear data analysis program BIAevaluation 3.2. All the experiments were done at 25°C.
Target
- Alternative Names
- Dengue virus
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for HPAB-AP059-YC. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone m366.6"
- CAT
- Product Name
See other products for "DENV"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-937 | Human Anti-DENV Recombinant Antibody (clone YC090); scFv Fragment | ELISA, PP, IF, Inhib | Human scFv |
| HPAB-0154CQ-S(P) | Recombinant Human Anti-DENV Antibody scFv Fragment (MAb 1A5) | ELISA, Neut | Human scFv |
| HPAB-1281LY-S(P) | Mouse Anti-DENV Recombinant Antibody; scFv Fragment (HPAB-1281LY-S(P)) | Neut, IHC, ELISA | Mouse scFv |
| HPAB-0128-YJ-S(P) | Human Anti-DENV Recombinant Antibody; scFv Fragment (HPAB-0128-YJ-S(P)) | ELISA, Neut, FuncS | Humanized scFv |
| HPAB-2089LY-S(P) | Mouse Anti-DENV Recombinant Antibody; scFv Fragment (HPAB-2089LY-S(P)) | ELISA, RIA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0154CQ | Recombinant Human Anti-DENV Antibody (MAb 1A5) | ELISA, Neut, WB, FuncS | Human IgG1 |
| HPAB-1281LY | Mouse Anti-DENV Recombinant Antibody (HPAB-1281LY) | Neut, IHC, ELISA | Mouse IgG |
| HPAB-2089LY | Mouse Anti-DENV Recombinant Antibody (HPAB-2089LY) | ELISA, RIA, WB | Mouse IgG |
| HPAB-AP058-YC | Recombinant Human Anti-DENV Antibody (m366) | SPR, ELISA, FC, Neut | |
| HPAB-AP060-YC | Recombinant Human Anti-DENV Antibody (m360) | SPR, ELISA, FC, Neut |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0128-YJ-F(E) | Human Anti-DENV Recombinant Antibody; Fab Fragment (HPAB-0128-YJ-F(E)) | ELISA, Neut, FuncS | Humanized Fab |
| HPAB-0129-YJ-F(E) | Human Anti-DENV Recombinant Antibody; Fab Fragment (HPAB-0129-YJ-F(E)) | ELISA, Neut, FuncS | Humanized Fab |
| HPAB-2089LY-F(E) | Mouse Anti-DENV Recombinant Antibody; Fab Fragment (HPAB-2089LY-F(E)) | ELISA, RIA, WB | Mouse Fab |
| HPAB-AP058-YC-F(E) | Recombinant Human Anti-DENV Antibody Fab Fragment (m366) | ELISA, FC, Neut | |
| HPAB-AP059-YC-F(E) | Recombinant Human Anti-DENV Antibody Fab Fragment (HPAB-AP059-YC-F(E)) | ELISA, FC, Neut |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, IF, FuncS
-2.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, FC, IF, WB
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, Inhib, FuncS
Application: ELISA, Inhib, FC, Neut
Application: ELISA, Cyt, PP, Inhib
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
