Recombinant Human Anti-DENV Antibody (m366.6) (CAT#: HPAB-AP059-YC)
Provided is a human anti-dengue virus antibody (an anti-DENV antibody) that binds to a DENV envelope protein and is cross-reactive with DENV serotype 1, DENV serotype 2, DENV serotype 3, and DENV serotype 4.
Specific Inquiry
SPR
Figure 1 Binding of m366.6 to DENV DIIIs from the four serotypes measured by Biacore.
The m366.6 was immobilized onto a CM5 chip, and the analytes consisted of serial dilution of DIII from DENV-1 (A), DENV-2 (B), DENV-3 (C), or DENV-4 (D). Binding kinetics was fitted using a 1:1 Langumir binding model by BIAevaluation 3.2 software.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Neut
Figure 2 eutralization and ADE effects of antibodies against different serotypes DENV.
DENV1-4 was mixed with 5-fold serial dilutions of mabs. Then neutralization activity was evaluated by plaque reduction assays in triplicates using BHK21 cells. Results were exhibited by percentage of plaques reduction and the independent neutralization experiments were performed in duplicate.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
FuncS
Figure 3 In vivo therapeutic efficacy of m366.6 against DENV1-4 infection.
Groups of one day old suckling mice were administrated with 100 μg of mAbs 4 hours after challenge with 200 PFU of DENV-1 (A), DENV-2 (B), DENV-3 (C), and DENV-4 (D), respectively. Unrelated antibody G12 was used in the negative controls. The number of animals for each group ranged from 6 to 9. Kaplan-Meier survival curves were analyzed by the log-rank test and compared to curves of the controls.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
ELISA
Figure 4 Binding of m366.6 to DENV DIII, ZIKV DIII, gp140 and PDL1 proteins measured by ELISA.
Binding of m366.6 to DENV DIII, ZIKV DIII, gp140 and PDL1 proteins measured by ELISA.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Specifications
- Host Species
- Human
- Derivation
- Human antibody library
- Specificity
- DENV
- Species Reactivity
- DENV
- Clone
- m366.6
- Applications
- SPR, Neut, ELISA, Funcs, FC
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Applications
- Application Notes
- The DENV antibody has been reported in applications of Surface Plasmon Resonance, Neutralization, Enzyme-linked Immunosorbent Assay, Functional Assay, Flow Cytometry.
For Surface Plasmon Resonance: Binding affinities of m360 scFv, m366 scFv, m360.6 scFv, and m366.6 scFv to the 4 DENV DIIIs were analyzed by surface plasmon resonance technology using a Biacore X100 instrument. The antibodies were covalently immobilized onto a sensor chip (CM5) using carbodiimide coupling chemistry. A control reference surface was prepared for nonspecific binding and refractive index changes. For analysis of the kinetics of interactions, varying concentrations of antigens were injected at flow rate of 30 μl/min using running buffer containing 10mM HEPES, 150 mM NaCl, 3 mM EDTA, and 0.05% Surfactant P-20 (pH 7.4). The association and dissociation phase data were fitted simultaneously to a 1:1 Langumir global model by using the nonlinear data analysis program BIAevaluation 3.2. All the experiments were done at 25°C.
Target
- Alternative Names
- Dengue virus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| MRO-419CQ | Recombinant Mouse Anti-DENV Antibody (MRO-419CQ) | Dot, ELISA, IF | Mouse antibody |
| MRO-420CQ | Recombinant Mouse Anti-DENV Antibody (D1-11(3)) | Dot, ELISA, IF, WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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