Human Anti-DIG Recombinant Antibody (clone DigB) (CAT#: FAMAB-0025JF)
Recombinant human anti-digoxigenin (DigB) is useful for patient monitoring and provide potential therapeutic reagents for neutralising overdoses.
Specific Inquiry
FC
Figure 1 FACS analysis using specific antibody against DIG.
Recombinant Y33N, Y33G, Y33S, expressing DIG antigen.
Daugherty, P. S., Chen, G., Olsen, M. J., Iverson, B. L., & Georgiou, G. (1998). Antibody affinity maturation using bacterial surface display. Protein engineering, 11(9), 825-832.
ELISA
Figure 2 Optical absorption spectrum of digoxigenin-BSA conjugate.
Molar ratio of hapten:carrier protein ¼ OD380 of conjugate/OD380 of carrier protein × 3.2.
Gill, P., Forouzandeh, M., Rahbarizadeh, F., Ramezani, R., & Javad Rasaee, M. (2006). Production of Anti‐Digoxigenin Antibody HRP Conjugate for PCR‐ELISA DIG Detection System. Journal of Immunoassay and Immunochemistry, 27(4), 303-318.
FuncS
Figure 3 Competitive standard curves of anti-DIG HRP conjugates.
Data pooled from two independent experiments (n = 5 + 5 per group).
Gill, P., Forouzandeh, M., Rahbarizadeh, F., Ramezani, R., & Javad Rasaee, M. (2006). Production of Anti‐Digoxigenin Antibody HRP Conjugate for PCR‐ELISA DIG Detection System. Journal of Immunoassay and Immunochemistry, 27(4), 303-318.
IHC
Figure 4 Dengue viral antigens detected in the liver in Kupffer and endothelial cells (arrow), but not in hepatocytes.
In situ hybridization, hematoxylin counterstain. Magnification, ×100.
Jessie, K., Fong, M. Y., Devi, S., Lam, S. K., & Wong, K. T. (2004). Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization. The Journal of infectious diseases, 189(8), 1411-1418.
Specifications
- Host Species
- Human
- Derivation
- Human phage display scFv library
- Type
- Human IgG
- Specificity
- Human digoxigenin
- Species Reactivity
- Human
- Clone
- DigB
- Applications
- ELISA, FuncS, FC, IHC
- Related Disease
- Neutralising overdoses
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The DIG antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Functional Assay, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- DIG; Digoxigenin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4567 | Hi-Affi™ Rabbit Anti-DIG Recombinant Antibody (clone TH77DS) | WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0025JF-F(E) | Human Anti-DIG Recombinant Antibody (clone DigB); Fab Fragment | WB, ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0025JF-S(P) | Human Anti-DIG Recombinant Antibody (clone DigB); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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