Rabbit Anti-DNM2 Recombinant Antibody (clone 1D8) (CAT#: ZG-0638J)
This product is a rabbit antibody that recognizes DNM2.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Rabbit Anti-DNM2 Antibody (ZG-0638J) in WB
Western Blot
Positive WB detected in: HL60 whole cell lysate, Rat brain tissue
All lanes: DNM2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 99, 98 kDa
Observed band size: 99 kDa
Figure 2 Rabbit Anti-DNM2 Antibody (ZG-0638J) in IF
Immunofluorescence staining of Hela Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Figure 3 Rabbit Anti-DNM2 Antibody (ZG-0638J) in FC
Overlay histogram showing Hela cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human Dynamin 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DNM2
- Species Reactivity
- Human, Rat
- Clone
- 1D8
- Applications
- ELISA, WB, IF, FC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Flow Cytometry.
WB:1:500-1:5000, IF:1:20-1:200, FC:1:20-1:200
Target
- Alternative Names
- DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB; dynamin-2; dynamin II
- Gene ID
- 1785
- UniProt ID
- P50570
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "DNM2"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2100MZ | Recombinant Mouse Anti-Human DNM2 Antibody (clone 6F5D3G4) | ELISA, IHC-Fr, IHC-P, WB | Mouse antibody |
MRO-0501-CN | Rabbit Anti-DNM2 Recombinant Antibody (clone JM61-38) | WB, IF, IHC, FC | Rabbit IgG |
ZG-0265J | Mouse Anti-DNM2 Recombinant Antibody (ZG-0265J) | WB, IHC, ELISA | Mouse IgG |
VS7-HM827 | Mouse Anti-DNM2 Recombinant Antibody (clone CBL202HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0638J. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA
Application: Neut, Inhib, FC, ELISA
Application: ELISA, Block, WB, FC, IP
Application: ELISA, IHC, IF, IP
Application: ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
For Research Use Only. Not For Clinical Use.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.