Rabbit Anti-DPF2 Recombinant Antibody (clone R39-2M-4) (CAT#: VS3-FY2168)
Clone R39-2M-4 is a rabbit IgG recombinant monoclonal antibody that reacts with human DPF2 protein, a calculated weight of 44 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R39-2M-4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200), immunoprecipitation (dilution range 1:50).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/if_selected.jpg
Figure 2 IHC staining of human salivary gland
(Immunohistochemical staining of human salivary gland shows weak cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_1_1_selected.jpg
(Immunohistochemical staining of human salivary gland shows weak cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_1_1_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_8_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_5.jpg
Figure 6 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_4_6.jpg
Figure 7 Skin
(Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_7_1.jpg
(Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_7_1.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133884-DPF2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133884-DPF2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DPF2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R39-2M-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44kDa.
- Related Disease
- Cancer
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Target
- Alternative Names
- Double PHD Fingers 2; D4, Zinc And Double PHD Fingers Family 2; Apoptosis Response Zinc Finger Protein; BRG1-Associated Factor 45D; Protein Requiem; BAF45D; UBID4
- Gene ID
- 5977
- UniProt ID
- Q92785
- Sequence Similarities
- Belongs to the requiem/DPF family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys59 and Lys108 (NX_Q92785 [NX_Q92785-1])
Modification sites at PhosphoSitePlus (Q92785)
Glycosylation from GlyGen (Q92785) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016873590.1; NP_001317237.1; NP_006259.1
- Function
- Plays an active role in transcriptional regulation by binding modified histones H3 and H4.
Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells.
Might also have a role in the development and maturation of lymphoid cells (By similarity).
Involved in the regulation of non-canonical NF-kappa-B pathway.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0597CT | Recombinant Mouse anti-Human DPF2 Monoclonal antibody (3394D2b) | Dot, WB |
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