Rabbit Anti-DPF2 Recombinant Antibody (clone R39-2M-4)
CAT#: VS3-FY2168
Clone R39-2M-4 is a rabbit IgG recombinant monoclonal antibody that reacts with human DPF2 protein, a calculated weight of 44 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R39-2M-4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200), immunoprecipitation (dilution range 1:50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows weak cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_1_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_4_6.jpg
Normal Tissue
Figure 7 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_7_1.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133884-DPF2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DPF2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R39-2M-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44kDa.
- Related Disease
- Cancer
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Target
- Alternative Names
- Double PHD Fingers 2; D4, Zinc And Double PHD Fingers Family 2; Apoptosis Response Zinc Finger Protein; BRG1-Associated Factor 45D; Protein Requiem; BAF45D; UBID4
- Gene ID
- 5977
- UniProt ID
- Q92785
- Sequence Similarities
- Belongs to the requiem/DPF family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys59 and Lys108 (NX_Q92785 [NX_Q92785-1])
Modification sites at PhosphoSitePlus (Q92785)
Glycosylation from GlyGen (Q92785) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016873590.1; NP_001317237.1; NP_006259.1
- Function
- Plays an active role in transcriptional regulation by binding modified histones H3 and H4.
Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells.
Might also have a role in the development and maturation of lymphoid cells (By similarity).
Involved in the regulation of non-canonical NF-kappa-B pathway.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MOR-1038 | Hi-Affi™ Rabbit Anti-DPF2 Recombinant Antibody (clone DS1038AB) | WB, IHC, ICC, IP | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY2061 | Anti-Mouse DPF2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2060 | Anti-Human DPF2 Immunohistochemistry Kit | IHC |
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