CytoStream™ Rabbit Anti-DROSHA Recombinant Antibody (VS13-YC324)
CAT#: VS13-YC324
The CytoStream™ Rabbit Anti-DROSHA Recombinant Antibody is specific for human DROSHA. DROSHA initiates microRNA synthesis, part of the microprocessor complex catalyzing pri-miRNA processing, with mutations affecting miRNA pathways and linked to cancer. The anti-DROSHA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31150/2013_C6_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113360-DROSHA
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Specifications
- Immunogen
- Recombinant human Drosha, aa 30-79.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DROSHA
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Drosha Ribonuclease III; Drosha, Double-Stranded RNA-Specific Endoribonuclease; Ribonuclease Type III, Nuclear; Drosha, Ribonuclease Type III; EC 3.1.26.3; RNase III; RNASE3L; RNASEN; P241; RN3;
- Gene ID
- 29102
- UniProt ID
- Q9NRR4
- Long Name
- Drosha Ribonuclease III
- Cellular Localization
- Nucleus
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DROSHA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1050 | Hi-Affi™ Rabbit Anti-DROSHA Recombinant Antibody (clone DS1050AB) | IHC-P, WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0496-CN | Rabbit Anti-DROSHA Recombinant Antibody (clone JU33-01) | WB, IF, IHC, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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