Rabbit Anti-DUT Recombinant Antibody (clone R89-6Y-1)
CAT#: VS3-XY3340
The antibody is a recombinant rabbit antibody targeting the human deoxyuridine triphosphatase (DUT), which inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR. This antibody specifically reacts with human DUT. It has been determined using Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/if_selected.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_B_6_4.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128951-DUT
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DUT
- Species Reactivity
- Human
- Clone
- R89-6Y-1
- Applications
- WB, ICC, IF, FC
- Related Disease
- Bone Marrow Failure; Diabetes Mellitus Syndrome; Cysticercosis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50
Target
- Alternative Names
- BMFDMS; dUTPase
- Gene ID
- 1854
- UniProt ID
- P33316
- Long Name
- Deoxyuridine triphosphatase
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys151, Lys179, and Lys251 (NX_P33316 [NX_P33316-3])
Modification sites at PhosphoSitePlus (P33316)
Glycosylation from GlyGen (P33316) 3 sites, 2 O-linked glycans (3 sites)
- Protein Refseq
- NP_001020419.1; NP_001020420.1
- Function
- Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DUT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3319z | Mouse Anti-DUT Recombinant Antibody (clone 1G5) | ELISA, IF, IHC, WB | Mouse IgG1, κ |
| MOB-2098MZ | Recombinant Mouse Anti-Human DUT Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| VS-0625-FY450 | Mouse Anti-dut Recombinant Antibody (clone 2506W0345) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0179MZ | Chicken Anti-DUT Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1067 | Hi-Affi™ Rabbit Anti-DUT Recombinant Antibody (clone DS1067AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY707 | Anti-DUT Immunohistochemistry Kit | IHC | |
| VS-0525-XY2115 | Anti-Human DUT Immunohistochemistry Kit | IHC |
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