Rabbit Anti-DUT Recombinant Antibody (clone R89-6Y-1) (CAT#: VS3-XY3340)
The antibody is a recombinant rabbit antibody targeting the human deoxyuridine triphosphatase (DUT), which inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR. This antibody specifically reacts with human DUT. It has been determined using Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50 and 1/50-1/200, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/if_selected.jpg
Figure 2 Bone marrow
(Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_B_6_4.jpg
(Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_B_6_4.jpg
Figure 3 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_7_8.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_7_3.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_7_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_8_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_8_4.jpg
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_9_5.jpg
Figure 7 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54422/123843_A_4_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128951-DUT
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128951-DUT
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DUT
- Species Reactivity
- Human
- Clone
- R89-6Y-1
- Applications
- WB, ICC, IF, FC
- Related Disease
- Bone Marrow Failure; Diabetes Mellitus Syndrome; Cysticercosis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50
Target
- Alternative Names
- BMFDMS; dUTPase
- Gene ID
- 1854
- UniProt ID
- P33316
- Long Name
- Deoxyuridine triphosphatase
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys151, Lys179, and Lys251 (NX_P33316 [NX_P33316-3])
Modification sites at PhosphoSitePlus (P33316)
Glycosylation from GlyGen (P33316) 3 sites, 2 O-linked glycans (3 sites)
- Protein Refseq
- NP_001020419.1; NP_001020420.1
- Function
- Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "DUT"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3319z | Mouse Anti-DUT Recombinant Antibody (clone 1G5) | ELISA, IF, IHC, WB | Mouse IgG1, κ |
| MOB-2098MZ | Recombinant Mouse Anti-Human DUT Antibody | ICC/IF, IHC-P, WB | Mouse antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0179MZ | Chicken Anti-DUT Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1067 | Hi-Affi™ Rabbit Anti-DUT Recombinant Antibody (clone DS1067AB) | WB, ICC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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