Mouse Anti-EIF2A Recombinant Antibody (EI934)
CAT#: VS-0326-YC934
The anti-EIF2A recombinant antibody (EI934) reacts with human, mouse, monkey and rat EIF2A. EIF2A is a translation initiation factor that can facilitate the binding of Met-tRNA to the 40S ribosomal subunit in a GTP-independent manner. While distinct from the core eIF2 complex, it is thought to play a specialized role in the translation of specific mRNAs during cellular stress when the standard eIF2 α-subunit is inhibited. The anti-EIF2A antibody (EI934) has been tested in Enzyme-linked Immunosorbent Assay (ELISA), Immunohistochemistry (IHC), Flow Cytometry (FC) and Western Blot (WB).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36256/392_H3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36256/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36256/71971_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36256/71971_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36256/71971_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36256/71971_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36256/71971_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000144895-EIF2A
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse, Monkey and Rat EIF2A
- Species Reactivity
- Human, Mouse, Monkey, Rat
- Clone
- EI934
- Applications
- ELISA, IHC, FC, WB
- Conjugate
- The unconjugated, Biotin and FITC formats are available.
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB (1/500-1/2000), IHC (1/200-1/1000), ELISA (1/5000-1/20000), FC (1/200-1/400)
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 2A; Eukaryotic Translation Initiation Factor 2A, 65 kDa; 5 KDa Eukaryotic Translation Initiation Factor 2A; EIF-2A; MSTP004; MSTP089; MST089; CDA02;
- Gene ID
- 83939
- UniProt ID
- Q9BY44
- Long Name
- Eukaryotic Translation Initiation Factor 2A
- Post Translation Modifications
- Unmodified
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS3-CJ408 | Mouse Anti-EIF2A Recombinant Antibody (VS3-CJ408) | WB |
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| VS3-XY601 | Mouse Anti-EIF2A Recombinant Antibody (clone 3A7A8) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS3-XY602 | Mouse Anti-EIF2A Recombinant Antibody (clone 3A7B11) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1136 | Rabbit Anti-EIF2A Recombinant Antibody (VS3-WK1136) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS3-FY481 | Recombinant Rabbit Anti-EIF2A Antibody (clone R08-8E2) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-1024-XY180 | Mouse Anti-NHP EIF2A Recombinant Antibody (clone 3A7B11) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2203 | Anti-EIF2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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