Rabbit Anti-EIF2AK3 Recombinant Antibody (VS3-FY2931) (CAT#: VS3-FY2931)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat EIF2AK3

• Species Reactivity
• Human, Mouse, Rat

• Applications
• Western Blot

• Conjugate
• Unconjugated, FITC

• Related Disease
• Epiphyseal Dysplasia, Multiple, With Early-Onset Diabetes Mellitus and Menkes Disease

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3; PRKR-Like Endoplasmic Reticulum Kinase; Pancreatic EIF2-Alpha Kinase; EC 2.7.11.1; PERK; PEK; Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3; HsPEK; WRS

• Gene ID
• 9451

• UniProt ID
• Q9NZJ5

• Sequence Similarities
• Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.

• Cellular Localization
• Endoplasmic reticulum membrane

• Post Translation Modifications
• Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop (By similarity).
  Autophosphorylated.
  Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity.
  Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity.
  (Q9NZJ5-E2AK3_HUMAN)
  N-glycosylated. (Q9NZJ5-E2AK3_HUMAN)
  ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity. (Q9NZJ5-E2AK3_HUMAN)
  Glycosylation at Asn258 (NX_Q9NZJ5 [NX_Q9NZJ5-1])
  Modification sites at PhosphoSitePlus (Q9NZJ5)
  Glycosylation from GlyGen (Q9NZJ5) 6 sites, 1 O-linked glycan (4 sites)

• Protein Refseq
• XP_047302385.1; XP_047302386.1; XP_054200611.1; XP_054200612.1

• Function
• Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability (By similarity).
  EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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