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  • Rabbit Anti-EIF3A Recombinant Antibody (clone R21-6S-3)

Rabbit Anti-EIF3A Recombinant Antibody (clone R21-6S-3) (CAT#: VS3-FY2197)

This R21-6S-3 recombinant monoclonal antibody is able to recognize human EIF3A protein, a calculated weight of 166 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R21-6S-3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).


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  • Gene Expression
  • Datasheet
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_B_9_5.jpg
Figure 3 Colon
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_7_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_4_6.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107581-EIF3A

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat EIF3A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R21-6S-3
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 166kDa, while the observed molecular weight stands at 160kDa.
  • Related Disease
  • Bardet-Biedl Syndrome

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Eukaryotic Translation Initiation Factor 3 Subunit A; Eukaryotic Translation Initiation Factor 3, Subunit 10 Theta, 150/170kDa; Eukaryotic Translation Initiation Factor 3 Subunit 10; EIF-3-Theta; EIF3 P167; EIF3 P180; EIF3 P185; EIF3S10; Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 150/170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10, 170kD; Eukaryotic Translation Initiation Factor 3, Subunit A; Cytoplasmic Protein P167
  • Sequence Similarities
  • Belongs to the eIF-3 subunit A family.
  • Cellular Localization
  • Cytoplasm
  • Post Translation Modifications
  • Phosphorylated.
    Phosphorylation is enhanced upon serum stimulation.
    (Q14152-EIF3A_HUMAN)
    Ubiquitination at Lys45, Lys13, Lys68, Lys75, Lys351, Lys409, Lys420, Lys672, Lys711, Lys775, and Lys777 (NX_Q14152 [NX_Q14152-1])
    Modification sites at PhosphoSitePlus (Q14152)
    Glycosylation from GlyGen (Q14152) 3 sites, 1 O-linked glycan (3 sites)
  • Function
  • The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "EIF3A"

Recombinant Antibody

CAT Product Name Application Type
MOB-2069z Mouse Anti-EIF3A Recombinant Antibody (clone 41G6) WB, ELISA, IF, IHC, IP Mouse IgG2b, κ
HPAB-2215LY Human Anti-EIF3A Recombinant Antibody (HPAB-2215LY) ELISA, WB, RIA, FC, IP, IHC Human IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1122 Hi-Affi™ Rabbit Anti-EIF3A Recombinant Antibody (clone DS1122AB) WB, IHC, ICC, FC Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-2215LY-F(E) Human Anti-EIF3A Recombinant Antibody; Fab Fragment (HPAB-2215LY-F(E)) ELISA, WB, RIA, FC, IP, IHC Human Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-2215LY-S(P) Human Anti-EIF3A Recombinant Antibody; scFv Fragment (HPAB-2215LY-S(P)) ELISA, WB, RIA, FC, IP, IHC Human scFv

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