Rabbit Anti-EIF3A Recombinant Antibody (clone R21-6S-3) (CAT#: VS3-FY2197)
This R21-6S-3 recombinant monoclonal antibody is able to recognize human EIF3A protein, a calculated weight of 166 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R21-6S-3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_B_9_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_7_5.jpg
Figure 6 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_4_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107581-EIF3A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107581-EIF3A
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EIF3A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R21-6S-3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 166kDa, while the observed molecular weight stands at 160kDa.
- Related Disease
- Bardet-Biedl Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 3 Subunit A; Eukaryotic Translation Initiation Factor 3, Subunit 10 Theta, 150/170kDa; Eukaryotic Translation Initiation Factor 3 Subunit 10; EIF-3-Theta; EIF3 P167; EIF3 P180; EIF3 P185; EIF3S10; Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 150/170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10, 170kD; Eukaryotic Translation Initiation Factor 3, Subunit A; Cytoplasmic Protein P167
- Gene ID
- 8661
- UniProt ID
- Q14152
- Sequence Similarities
- Belongs to the eIF-3 subunit A family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated.
Phosphorylation is enhanced upon serum stimulation.
(Q14152-EIF3A_HUMAN)
Ubiquitination at Lys45, Lys13, Lys68, Lys75, Lys351, Lys409, Lys420, Lys672, Lys711, Lys775, and Lys777 (NX_Q14152 [NX_Q14152-1])
Modification sites at PhosphoSitePlus (Q14152)
Glycosylation from GlyGen (Q14152) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_003741.1
- Function
- The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2069z | Mouse Anti-EIF3A Recombinant Antibody (clone 41G6) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| HPAB-2215LY | Human Anti-EIF3A Recombinant Antibody (HPAB-2215LY) | ELISA, WB, RIA, FC, IP, IHC | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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