Rabbit Anti-EIF3A Recombinant Antibody (clone R21-6S-3)
CAT#: VS3-FY2197
This R21-6S-3 recombinant monoclonal antibody is able to recognize human EIF3A protein, a calculated weight of 166 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R21-6S-3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38316/79106_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107581-EIF3A
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EIF3A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R21-6S-3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 166kDa, while the observed molecular weight stands at 160kDa.
- Related Disease
- Bardet-Biedl Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 3 Subunit A; Eukaryotic Translation Initiation Factor 3, Subunit 10 Theta, 150/170kDa; Eukaryotic Translation Initiation Factor 3 Subunit 10; EIF-3-Theta; EIF3 P167; EIF3 P180; EIF3 P185; EIF3S10; Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 150/170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10, 170kD; Eukaryotic Translation Initiation Factor 3, Subunit A; Cytoplasmic Protein P167
- Gene ID
- 8661
- UniProt ID
- Q14152
- Sequence Similarities
- Belongs to the eIF-3 subunit A family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated.
Phosphorylation is enhanced upon serum stimulation.
(Q14152-EIF3A_HUMAN)
Ubiquitination at Lys45, Lys13, Lys68, Lys75, Lys351, Lys409, Lys420, Lys672, Lys711, Lys775, and Lys777 (NX_Q14152 [NX_Q14152-1])
Modification sites at PhosphoSitePlus (Q14152)
Glycosylation from GlyGen (Q14152) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_003741.1
- Function
- The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2069z | Mouse Anti-EIF3A Recombinant Antibody (clone 41G6) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| HPAB-2215LY | Human Anti-EIF3A Recombinant Antibody (HPAB-2215LY) | ELISA, WB, RIA, FC, IP, IHC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1122 | Hi-Affi™ Rabbit Anti-EIF3A Recombinant Antibody (clone DS1122AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| HPAB-2215LY-F(E) | Human Anti-EIF3A Recombinant Antibody; Fab Fragment (HPAB-2215LY-F(E)) | ELISA, WB, RIA, FC, IP, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2215LY-S(P) | Human Anti-EIF3A Recombinant Antibody; scFv Fragment (HPAB-2215LY-S(P)) | ELISA, WB, RIA, FC, IP, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY743 | Anti-EIF3A Immunohistochemistry Kit | IHC | |
| VS-0525-XY2217 | Anti-Mouse EIF3A Immunohistochemistry Kit | IHC | |
| VS-0525-XY2216 | Anti-Human EIF3A Immunohistochemistry Kit | IHC |
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