CytoStream™ Rabbit Anti-EIF4EBP1 Recombinant Antibody (VS13-YC351) (CAT#: VS13-YC351)
The CytoStream™ Rabbit Anti-EIF4EBP1 Recombinant Antibody is specific for human, mouse and rat EIF4EBP1. EIF4EBP1 acts as a translational repressor, inhibiting complex assembly through interactions with EIF4E, influenced by phosphorylation signaling to derepress mRNA translation. The anti-EIF4EBP1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/628_E3_3_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/628_E3_3_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5039/630_D3_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5039/630_D3_1_selected.jpg
Figure 3 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_8_5.jpg
Figure 4 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_7_8.jpg
Figure 6 Salivary gland
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_B_3_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_B_3_1.jpg
Figure 7 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_8_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5032/15087_A_8_4.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187840-EIF4EBP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187840-EIF4EBP1
Specifications
- Immunogen
- Recombinant human eIF4EBP1, aa 1-49.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EIF4EBP1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 4E Binding Protein 1; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1; EIF4E-Binding Protein 1; 4E-BP1; PHAS-I; Eukaryotic Translation Initiation Factor 4E-Binding Protein 1; 4EBP1; BP-1;
- Gene ID
- 1978
- UniProt ID
- Q13541
- Long Name
- Eukaryotic Translation Initiation Factor 4E Binding Protein 1
- Cellular Localization
- Cytoplasm; Cytosol
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Insulin Signaling Pathway
mTOR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2163z | Mouse Anti-EIF4EBP1 Recombinant Antibody (clone 35A12) | WB, ELISA, IF, IHC | Mouse IgG2b |
| VS3-FY492 | Recombinant Rabbit Anti-EIF4EBP1 Antibody (clone R06-8E6) | WB, IHC-P, IP | Rabbit IgG |
| VS7-HM852 | Mouse Anti-EIF4EBP1 Recombinant Antibody (clone CBL222HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| VS7-HM853 | Mouse Anti-EIF4EBP1 Recombinant Antibody (clone CBL223HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| VS7-HM854 | Mouse Anti-EIF4EBP1 Recombinant Antibody (clone CBL224HM) | WB, ELISA | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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