Mouse Anti-EIF5 Recombinant Antibody (EI527)
CAT#: VS-0326-YC527
The anti-EIF5 recombinant antibody (EI527) reacts with human and monkey EIF5. EIF5 is a GTPase-activating protein (GAP) essential for the final step of translation initiation. It promotes the hydrolysis of GTP bound to eIF2 within the 48S pre-initiation complex. This action triggers the release of initiation factors and allows the joining of the 60S ribosomal subunit to form a functional 80S ribosome ready for elongation. The anti-EIF5 antibody (EI527) has been tested in Enzyme-linked Immunosorbent Assay (ELISA) and Western Blot (WB).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4226/671_B2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/867/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4226/12204_A_9_8.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4226/12204_B_3_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4226/12204_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4226/12204_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4226/12204_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100664-EIF5
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human and Monkey EIF5
- Species Reactivity
- Human, Monkey
- Clone
- EI527
- Applications
- ELISA, WB
- Conjugate
- The unconjugated, Biotin and FITC formats are available.
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB (1/500-1/2000), ELISA (1/5000-1/20000)
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EIF5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1378MZ | Recombinant Mouse Anti-Human EIF5 Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1132 | Hi-Affi™ Rabbit Anti-EIF5 Recombinant Antibody (clone DS1132AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK172 | Mouse Anti-EIF5 Recombinant Antibody (clone H6-D11) | WB, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY184 | Mouse Anti-NHP EIF5 Recombinant Antibody (clone 6D6G6) | WB, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-HM855 | Mouse Anti-EIF5 Recombinant Antibody (clone 6D6H11) | IHC, ICC, FCM | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY756 | Anti-EIF5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2236 | Anti-Human EIF5 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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