What applications is the Mouse IgG2a, kappa Isotype Control Antibody suitable for?

The Mouse IgG2a, kappa Isotype Control Antibody is primarily used in flow cytometry, immunohistochemistry, and ELISA. It serves as a control to ensure the specificity of primary antibody binding without interference.

What makes this antibody suitable as an experimental control?

Its class and subclass match many primary antibodies, providing an optimal control to help discern background from specific signal in experiments.

In which species is it successfully tested?

It is widely tested on mouse and human samples, guaranteeing control across multiple species for consistency.

Is this antibody compatible with fluorescent secondary antibodies?

Yes, it's highly compatible with most fluorescent labeled secondary antibodies, making it useful in fluorescence-based assays.

Is there a recommended dilution factor for this antibody?

Typically, a 1:100 to 1:1000 range, depending on your primary antibody concentration and the experimental platform.

Mouse IgG2a, kappa Isotype Control Antibody

Specifications

Host Species
Mouse

Type
Mouse IgG2a, κ

Applications
Used for immunoassay techniques such as: Flow Cytometry; Intracellular Staining for Flow Cytometry; Western Blot; Immunoprecipitation; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Functional Studies

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration

Buffer
PBS

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Customer Reviews and Q&As

Customer Review Q&As

Vital for Accurate Results

As a researcher focused on immunology, the use of this isotype control has been transformative. It efficiently differentiates between specific and non-specific staining, particularly in flow cytometry. This has elevated the quality and accuracy of our data significantly.

High Compatibility with Fluorescence Assays

The compatibility of this isotype control with fluorescent antibodies is commendable. It has seamlessly integrated into our fluorescence microscopy set-up, reducing background noise and enhancing signal specificity.

A Must for Consistency

The consistency it brings to both my ELISA and flow cytometry results is unmatched. By ruling out false positives, it raises the standard of experimental evidence I can present.

Multipurpose Utility

Its ability to be used across different assays like ELISA or Western blotting is impressive. It adds great versatility for assorted immunological experiments.

Streamlined Protocol Setup

The straightforward protocols and guidelines for this product simplify its deployment in complex experimental setups, reducing potential errors and improving results.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MOB-202CQ, RRID: AB_3111496)