Mouse IgG2a, kappa Isotype Control Antibody
CAT#: MOB-202CQ
This product is a Mouse IgG2a, kappa isotype control antibody and it can also be used as a negative control in various diagnostic applications such as FC, ICFC, WB, IP, ICC, IF, IHC, FuncS.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Intracellular Staining for Flow Cytometry; Western Blot; Immunoprecipitation; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Functional Studies
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Mouse IgG2a, kappa Isotype Control; Mouse IgG2a, kappa negative control
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Vital for Accurate Results
As a researcher focused on immunology, the use of this isotype control has been transformative. It efficiently differentiates between specific and non-specific staining, particularly in flow cytometry. This has elevated the quality and accuracy of our data significantly.
High Compatibility with Fluorescence Assays
The compatibility of this isotype control with fluorescent antibodies is commendable. It has seamlessly integrated into our fluorescence microscopy set-up, reducing background noise and enhancing signal specificity.
A Must for Consistency
The consistency it brings to both my ELISA and flow cytometry results is unmatched. By ruling out false positives, it raises the standard of experimental evidence I can present.
Multipurpose Utility
Its ability to be used across different assays like ELISA or Western blotting is impressive. It adds great versatility for assorted immunological experiments.
Streamlined Protocol Setup
The straightforward protocols and guidelines for this product simplify its deployment in complex experimental setups, reducing potential errors and improving results.
Q&As
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What applications is the Mouse IgG2a, kappa Isotype Control Antibody suitable for?
A: The Mouse IgG2a, kappa Isotype Control Antibody is primarily used in flow cytometry, immunohistochemistry, and ELISA. It serves as a control to ensure the specificity of primary antibody binding without interference.
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What makes this antibody suitable as an experimental control?
A: Its class and subclass match many primary antibodies, providing an optimal control to help discern background from specific signal in experiments.
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In which species is it successfully tested?
A: It is widely tested on mouse and human samples, guaranteeing control across multiple species for consistency.
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Is this antibody compatible with fluorescent secondary antibodies?
A: Yes, it's highly compatible with most fluorescent labeled secondary antibodies, making it useful in fluorescence-based assays.
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Is there a recommended dilution factor for this antibody?
A: Typically, a 1:100 to 1:1000 range, depending on your primary antibody concentration and the experimental platform.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOB-202CQ, RRID: AB_3111496)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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