Rabbit Anti-ELP4 Recombinant Antibody (clone R37-8N-5) (CAT#: VS3-FY2162)
Clone R37-8N-5 is a rabbit IgG recombinant monoclonal antibody that is able to recognize human ELP4 protein, a calculated weight of 46 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R37-8N-5 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/if_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_B_8_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_B_8_5.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_8_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_8_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_9_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_6_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_6_6.jpg
Figure 7 Lymph node
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_9_8.jpg
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109911-ELP4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109911-ELP4
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ELP4
- Species Reactivity
- Human, Mouse
- Clone
- R37-8N-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 46 kDa, while the observed molecular weight stands at 46kDa.
- Related Disease
- Aniridia 2 and Coloboma Of Optic Nerve
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Elongator Acetyltransferase Complex Subunit 4; PAX6 Neighbor Gene Protein; C11orf19; PAXNEB; HELP4; Elongation Protein 4 Homolog (S. Cerevisiae); Chromosome 11 Open Reading Frame 19
- Gene ID
- 26610
- UniProt ID
- Q96EB1
- Sequence Similarities
- Belongs to the ELP4 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q96EB1)
Modification sites at neXtProt (NX_Q96EB1)
Glycosylation from GlyGen (Q96EB1) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001275654.1; NP_001275655.1; NP_061913.3
- Function
- Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine).
The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Please select product type
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1142 | Hi-Affi™ Rabbit Anti-ELP4 Recombinant Antibody (clone DS1142AB) | WB, IHC, ICC | Rabbit IgG |
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