Rabbit Anti-ELP4 Recombinant Antibody (clone R37-8N-5)
CAT#: VS3-FY2162
Clone R37-8N-5 is a rabbit IgG recombinant monoclonal antibody that is able to recognize human ELP4 protein, a calculated weight of 46 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R37-8N-5 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38572/81714_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109911-ELP4
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ELP4
- Species Reactivity
- Human, Mouse
- Clone
- R37-8N-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 46 kDa, while the observed molecular weight stands at 46kDa.
- Related Disease
- Aniridia 2 and Coloboma Of Optic Nerve
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Elongator Acetyltransferase Complex Subunit 4; PAX6 Neighbor Gene Protein; C11orf19; PAXNEB; HELP4; Elongation Protein 4 Homolog (S. Cerevisiae); Chromosome 11 Open Reading Frame 19
- Gene ID
- 26610
- UniProt ID
- Q96EB1
- Sequence Similarities
- Belongs to the ELP4 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q96EB1)
Modification sites at neXtProt (NX_Q96EB1)
Glycosylation from GlyGen (Q96EB1) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001275654.1; NP_001275655.1; NP_061913.3
- Function
- Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine).
The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ELP4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1142 | Hi-Affi™ Rabbit Anti-ELP4 Recombinant Antibody (clone DS1142AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2263 | Anti-ELP4 Immunohistochemistry Kit | IHC |
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