Anti-EPHB4 Antibody Prodrug, Protease Activated (22E6)
CAT#: VS-1025-YC121
The Anti-EPHB4 Antibody Prodrug, Protease Activated (22E6) is an antibody prodrug comprised of a recombinant human antibody targeting human EPHB4, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to EPHB4 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to EPHB4 effectively. EPHB4 (Ephrin Receptor B4) is a receptor tyrosine kinase that is frequently overexpressed and often associated with promoting tumor angiogenesis and survival in a variety of solid tumors, including breast and prostate cancer. Its role in promoting cell adhesion and vascular maturation makes it a potential therapeutic target.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_4_6.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_1_7.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196411-EPHB4
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Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human EPHB4
- Target
- EPHB4
- Species Reactivity
- Human
- Isotype
- IgG1, kappa
- Clone
- 22E6
- Affinity Constant
- KD = 1.9 nM
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
- Alternative Names
- EPH Receptor B4; Tyrosine-Protein Kinase TYRO11; EC 2.7.10.1; TYRO11; MYK1; HTK; EC 2.7.10; EphB4
- Gene ID
- 2050
- UniProt ID
- P54760
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Datasheet
MSDS
COA
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See other products for "Clone 22E6"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2116z | Mouse Anti-EPHB4 Recombinant Antibody (clone 25G10) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| HPAB-0184-CN | Human Anti-EPHB4 Recombinant Antibody (clone 22E6) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0185-CN | Human Anti-EPHB4 Recombinant Antibody (clone 30C2) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0186-CN | Human Anti-EPHB4 Recombinant Antibody (clone 1E10) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0187-CN | Human Anti-EPHB4 Recombinant Antibody (clone 4F2) | ELISA, WB, IP | Humanized IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-001WM | Anti-Human EPHB4 Recombinant Antibody (MOR03640) | ELISA, FC | Human Antibody |
| TAB-002WM | Anti-Human EPHB4 Recombinant Antibody (TAB-002WM) | ELISA | Human Antibody |
| TAB-001WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (MOR03640) | ELISA, FC | Human Antibody |
| TAB-002WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (TAB-002WM-S(P)) | ELISA | Human Antibody |
| TAB-001WM-F(E) | Anti-Human EPHB4 Recombinant Antibody Fab Fragment (MOR03640) | ELISA, FC | Human Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-003WM | Mouse Anti-EPHB4 Recombinant Antibody (TAB-003WM) | IHC, WB, FC | Mouse IgG2b |
| TAB-003WM-S(P) | Mouse Anti-EPHB4 Recombinant Antibody; scFv Fragment (TAB-003WM-S(P)) | IHC, WB | Mouse scFv |
| TAB-003WM-F(E) | Mouse Anti-EPHB4 Recombinant Antibody; Fab Fragment (TAB-003WM-F(E)) | IHC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1169 | Hi-Affi™ Rabbit Anti-EPHB4 Recombinant Antibody (clone DS1169AB), FITC | FC | Rabbit IgG |
| MOR-4157 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI232DS) | FC | Rabbit IgG |
| MOR-4158 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI233DS) | ELISA, FC | Rabbit IgG |
| MOR-4159 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI234DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0185-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0186-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0187-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-1128-FY-F(E) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0185-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0186-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0187-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-1128-FY-S(P) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY89 | AbPlus™ Anti-EPHB4 Magnetic Beads (22E6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY774 | Anti-EPHB4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2318 | Anti-Mouse EPHB4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2317 | Anti-Human EPHB4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC372 | Recombinant Anti-EPHB4 Vesicular Antibody, EV Displayed (VS-0425-YC372) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC116 | SmartAb™ Recombinant Anti-EPHB4 pH-dependent Antibody (Clone 22E6) | ELISA, WB, IP | Human IgG1 kappa |
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