Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI233DS)
CAT#: MOR-4158
Recombinant Rabbit anti-EphB4 monoclonal antibody for ELISA, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_4_6.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_1_7.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196411-EPHB4
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Specifications
- Immunogen
- Recombinant Human EphB4/Eph Receptor B4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EphB4
- Species Reactivity
- Human
- Clone
- SI233DS
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay (0.1-0.2 μg/ml), Flow Cytometry (0.5-2 μg/Test).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-001WM | Anti-Human EPHB4 Recombinant Antibody (MOR03640) | ELISA, FC | Human Antibody |
| TAB-001WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (MOR03640) | ELISA, FC | Human Antibody |
| TAB-002WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (TAB-002WM-S(P)) | ELISA | Human Antibody |
| TAB-001WM-F(E) | Anti-Human EPHB4 Recombinant Antibody Fab Fragment (MOR03640) | ELISA, FC | Human Antibody |
| TAB-002WM-F(E) | Anti-Human EPHB4 Recombinant Antibody Fab Fragment (TAB-002WM-F(E)) | ELISA | Human Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-003WM | Mouse Anti-EPHB4 Recombinant Antibody (TAB-003WM) | IHC, WB, FC | Mouse IgG2b |
| TAB-003WM-S(P) | Mouse Anti-EPHB4 Recombinant Antibody; scFv Fragment (TAB-003WM-S(P)) | IHC, WB | Mouse scFv |
| TAB-003WM-F(E) | Mouse Anti-EPHB4 Recombinant Antibody; Fab Fragment (TAB-003WM-F(E)) | IHC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1169 | Hi-Affi™ Rabbit Anti-EPHB4 Recombinant Antibody (clone DS1169AB), FITC | FC | Rabbit IgG |
| MOR-4157 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI232DS) | FC | Rabbit IgG |
| MOR-4159 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI234DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184-CN | Human Anti-EPHB4 Recombinant Antibody (clone 22E6) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0185-CN | Human Anti-EPHB4 Recombinant Antibody (clone 30C2) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0186-CN | Human Anti-EPHB4 Recombinant Antibody (clone 1E10) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0187-CN | Human Anti-EPHB4 Recombinant Antibody (clone 4F2) | ELISA, WB, IP | Humanized IgG1, κ |
| VS3-XY636 | Mouse Anti-EPHB4 Recombinant Antibody (clone 5B5) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0185-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0186-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0187-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-1128-FY-F(E) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0185-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0186-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0187-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-1128-FY-S(P) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY89 | AbPlus™ Anti-EPHB4 Magnetic Beads (22E6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY774 | Anti-EPHB4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2318 | Anti-Mouse EPHB4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2317 | Anti-Human EPHB4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC372 | Recombinant Anti-EPHB4 Vesicular Antibody, EV Displayed (VS-0425-YC372) | ELISA, FC, Cell-uptake |
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