This product was isolated from the HuCAL GOLD® antibody library. This product cross-reactive to both the murine and the human EphB4 extracellular domain, and its use as medicament for the treatment of pathological angiogenesis potentially.
Figure 1 The specificity of Fabs for EphB4 and cross-reactivity to both human and murine EphB4 in ELISA.
Anti-EphB4 Fab fragments (purified, MH-tagged) were added (100 uL/well, 100 g/mL final concentration in chemiblock) to respective wells and incubated for 1 h at RT.Then 100 ul mEphrinB2-Fc fusion protein either spiked with the respective anti-EphB4 Fab or with PBS was added (25 ng/ml mEphrinB2+/-100 ug/ml Fab diluted in chemiblock)and incubated for max. 20 min at RT. The amount of bound EphrinB2 was detected using biotinylated goat anti-EphrinB2 antibody (0.05 g/ml final concentration diluted in chemiblock, 1 h at RT), AP-conjugated streptavidin and AttoPhos substrate.
Figure 2 The FACS analysis of MCF-7 breast cancer cell line with anti-EphB4 Fabs.
FACS analysis was used to confirm that the MCF-7 breast cancer cell line is highly positive for EphB4 expres sion. EphB4 Fabs were incubated at a concentration of 10 ug/mL with MCF-7 cells at 4°C. for 1 hour. Afterwashing with PBS, the cells were incubated with FITC-labelled sec ondary antibody. After a final PBS wash, the cell-associated fluorescence was measured using a Becton Dickinson Calibur Fluorescence activated cell sorter.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-002WM | Anti-Human EPHB4 Recombinant Antibody (30.35) | ELISA | Human Antibody |
TAB-001WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (MOR03640) | ELISA, FC | Human Antibody |
TAB-002WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (30.35) | ELISA | Human Antibody |
TAB-001WM-F(E) | Anti-Human EPHB4 Recombinant Antibody Fab Fragment (MOR03640) | ELISA, FC | Human Antibody |
TAB-002WM-F(E) | Anti-Human EPHB4 Recombinant Antibody Fab Fragment (30.35) | ELISA | Human Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-003WM | Mouse Anti-EPHB4 Recombinant Antibody (TAB-003WM) | IHC, WB, FC | Mouse IgG2b |
TAB-003WM-S(P) | Mouse Anti-EPHB4 Recombinant Antibody; scFv Fragment (TAB-003WM-S(P)) | IHC, WB | Mouse scFv |
TAB-003WM-F(E) | Mouse Anti-EPHB4 Recombinant Antibody; Fab Fragment (TAB-003WM-F(E)) | IHC, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1169 | Hi-Affi™ Rabbit Anti-EPHB4 Recombinant Antibody (clone DS1169AB), FITC | FC | Rabbit IgG |
MOR-4157 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI232DS) | FC | Rabbit IgG |
MOR-4158 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI233DS) | ELISA, FC | Rabbit IgG |
MOR-4159 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI234DS) | ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0184-CN | Human Anti-EPHB4 Recombinant Antibody (clone 22E6) | ELISA, WB, IP | Humanized IgG1, κ |
HPAB-0185-CN | Human Anti-EPHB4 Recombinant Antibody (clone 30C2) | ELISA, WB, IP | Humanized IgG1, κ |
HPAB-0186-CN | Human Anti-EPHB4 Recombinant Antibody (clone 1E10) | ELISA, WB, IP | Humanized IgG1, κ |
HPAB-0187-CN | Human Anti-EPHB4 Recombinant Antibody (clone 4F2) | ELISA, WB, IP | Humanized IgG1, κ |
HPAB-1128-FY | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265) | ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0184-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); scFv Fragment | ELISA, WB, IP | Humanized scFv |
HPAB-0185-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
HPAB-0186-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); scFv Fragment | ELISA, WB, IP | Humanized scFv |
HPAB-0187-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
HPAB-1128-FY-F(E) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); scFv Fragment | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0184-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); Fab Fragment | ELISA, WB, IP | Humanized Fab |
HPAB-0185-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
HPAB-0186-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); Fab Fragment | ELISA, WB, IP | Humanized Fab |
HPAB-0187-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
HPAB-1128-FY-S(P) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); Fab Fragment | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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