Rabbit Anti-EPHX2 Recombinant Antibody (clone R04-2F6)
CAT#: VS3-FY2001
Clone R04-2F6 is a rabbit recombinant monoclonal antibody that is designed to recognize human EPHX2. It cross-reacts with mouse. This epoxide hydrolase 1 antibody is guaranteed for western blot analysis of EPHX2 in mouse liver lysates and western blot analysis of EPHX2 in A549, HL-60, U251, U87-MG lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23094/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23094/52398_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23094/52398_A_4_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23094/52398_A_9_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23094/52398_A_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (JURKAT, K-562, OE19)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000120915-EPHX2
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WB
Figure 1 Anti-EPHX2 Antibody (VS3-FY2001) in WB.
The EPHX2 expression in mouse liver lysates were detected using the anti-Epoxide Hydrolase 1 by western blot assay.
WB
Figure 2 Anti-EPHX2 Antibody (VS3-FY2001) in WB.
The EPHX2 expression in A549, HL-60, U251, U87-MG lysates were detected using the anti-EPHX2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human EPHX2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse EPHX2
- Species Reactivity
- Human, Mouse
- Clone
- R04-2F6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 63 kDa, while the observed molecular weight stands at 63 kDa.
- Related Disease
- Hypercholesterolemia, Familial, 1 and Familial Hypercholesterolemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH
- Gene ID
- 2053
- UniProt ID
- P34913
- Sequence Similarities
- Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
- Cellular Localization
- Cytoplasm, Peroxisome
- Post Translation Modifications
- The N-terminus is blocked. (P34913-HYES_HUMAN)
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible.
It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).
(P34913-HYES_HUMAN)
Ubiquitination at Lys84, Lys89, Lys43, Lys174, Lys191, Lys245, Lys406, Lys421, Lys456, Lys474, and Lys530 (NX_P34913 [NX_P34913-1])
Modification sites at PhosphoSitePlus (P34913)
Glycosylation from GlyGen (P34913) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001243411.1; NP_001243412.1; NP_001243413.1; NP_001400945.1; NP_001400946.1
- Function
- Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity).
Also determines steady-state levels of physiological mediators.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "EPHX2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1170 | Hi-Affi™ Rabbit Anti-EPHX2 Recombinant Antibody (clone DS1170AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY775 | Anti-EPHX2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2323 | Anti-Mouse EPHX2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2322 | Anti-Human EPHX2 Immunohistochemistry Kit | IHC |
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