Rabbit Anti-EPHX2 Recombinant Antibody (clone R04-2F6) (CAT#: VS3-FY2001)
Clone R04-2F6 is a rabbit recombinant monoclonal antibody that is designed to recognize human EPHX2. It cross-reacts with mouse. This epoxide hydrolase 1 antibody is guaranteed for western blot analysis of EPHX2 in mouse liver lysates and western blot analysis of EPHX2 in A549, HL-60, U251, U87-MG lysates.
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WB
Figure 1 Anti-EPHX2 Antibody (VS3-FY2001) in WB.
The EPHX2 expression in mouse liver lysates were detected using the anti-Epoxide Hydrolase 1 by western blot assay.
WB
Figure 2 Anti-EPHX2 Antibody (VS3-FY2001) in WB.
The EPHX2 expression in A549, HL-60, U251, U87-MG lysates were detected using the anti-EPHX2 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human EPHX2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse EPHX2
- Species Reactivity
- Human, Mouse
- Clone
- R04-2F6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 63 kDa, while the observed molecular weight stands at 63 kDa.
- Related Disease
- Hypercholesterolemia, Familial, 1 and Familial Hypercholesterolemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH
- Gene ID
- 2053
- UniProt ID
- P34913
- Sequence Similarities
- Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
- Cellular Localization
- Cytoplasm, Peroxisome
- Post Translation Modifications
- The N-terminus is blocked. (P34913-HYES_HUMAN)
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible.
It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).
(P34913-HYES_HUMAN)
Ubiquitination at Lys84, Lys89, Lys43, Lys174, Lys191, Lys245, Lys406, Lys421, Lys456, Lys474, and Lys530 (NX_P34913 [NX_P34913-1])
Modification sites at PhosphoSitePlus (P34913)
Glycosylation from GlyGen (P34913) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001243411.1; NP_001243412.1; NP_001243413.1; NP_001400945.1; NP_001400946.1
- Function
- Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity).
Also determines steady-state levels of physiological mediators.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1170 | Hi-Affi™ Rabbit Anti-EPHX2 Recombinant Antibody (clone DS1170AB) | WB, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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