Mouse Anti-ETS1 Recombinant Antibody (VS3-CJ658) (CAT#: VS3-CJ658)
This product is a mouse antibody that recognizes human ETS1.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63230/1151_D9_1_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63230/1151_D9_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/if_selected.jpg
Figure 3 High expression in lymph node.
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_8_8.jpg
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_8_8.jpg
Figure 4 Tonsil
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_5_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_5_8.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEL, JURKAT, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134954-ETS1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134954-ETS1
Specifications
- Immunogen
- Recombinant protein of human ETS1
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human ETS1
- Species Reactivity
- Human
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500), Flow Cytometry (1:25).
Target
- Alternative Names
- p54; ETS-1; EWSR2; c-ets-1
- Gene ID
- 2113
- UniProt ID
- P14921
- Sequence Similarities
- Belongs to the ETS family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
Phosphorylation at Ser-251, Ser-282 and Ser-285 by CaMK2/CaMKII in response to calcium signaling decreases affinity for DNA: an increasing number of phosphoserines causes DNA-binding to become progressively weaker.
- Protein Refseq
- NP_001137292.1; NP_001155894.1; NP_001317380.1
- Function
- Transcription factor (PubMed:10698492, PubMed:11909962).
Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371).
May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371).
May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3991z | Mouse Anti-ETS1 Recombinant Antibody (clone 25F12) | ELISA, FC, IF, IHC, WB | Mouse IgG1 |
| MOB-2368MZ | Recombinant Mouse Anti-Human ETS1 Antibody (clone 2H22) | IHC-P, IP, WB | Mouse antibody |
| ZG-0324J | Mouse Anti-ETS1 Recombinant Antibody (ZG-0324J) | WB, IHC, ELISA | Mouse IgG |
| VS3-QX392 | Mouse Anti-ETS1 Recombinant Antibody (clone 8A8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY524 | Recombinant Rabbit Anti-ETS1 Antibody (clone R04-8C7) | WB | Rabbit IgG |
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