Anti-FAT1 Antibody Prodrug, Protease Activated (VS-1025-YC88)
CAT#: VS-1025-YC88
The Anti-FAT1 Antibody Prodrug, Protease Activated (VS-1025-YC88) is an antibody prodrug comprised of a recombinant mouse antibody targeting human FAT1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to FAT1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to FAT1 effectively. FAT1 is a huge protocadherin that functions as a major tumor suppressor involved in cell adhesion, polarity, and regulating the Hippo signaling pathway. Loss-of-function mutations or deletions in FAT1 are highly recurrent across many cancers, including head and neck squamous cell carcinoma and leukemia, where its inactivation promotes abnormal cell proliferation and invasion.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23882/52199_A_5_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1869/7555_B_8_5.jpg
Normal Tissue
Figure 3 Small intestine
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1869/7834_A_6_2.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1869/7834_A_7_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (fHDF/TERT166, LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000083857-FAT1
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Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human FAT1
- Target
- FAT1
- Species Reactivity
- Human
- Isotype
- IgG2a, kappa
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described.
- Alternative Names
- FAT; ME5; CDHF7; CDHR8; hFat1
- Gene ID
- 2195
- UniProt ID
- Q14517
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Datasheet
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COA
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See other products for "FAT1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4061z | Mouse Anti-FAT1 Recombinant Antibody (clone 44H5) | WB, ELISA | Mouse IgG1, κ |
| MRO-2136-CN | Rabbit Anti-FAT1 Polyclonal Antibody (MRO-2136-CN) | WB | Rabbit IgG |
| HPAB-0124-WJ | Mouse Anti-FAT1 Recombinant Antibody (HPAB-0124-WJ) | IHC, ELISA, WB | Mouse IgG2a, κ |
| VS3-WK959 | Mouse Anti-FAT1 Recombinant Antibody (VS3-WK959) | WB | Mouse IgG |
| MOB-328YC | Rabbit Anti-FAT1 Recombinant Antibody (MOB-328YC) | ELISA, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-030WM | Anti-Human FAT1 Recombinant Antibody (198.3) | FC, WB, IHC | |
| TAB-030WM-S(P) | Anti-Human FAT1 Recombinant Antibody scFv Fragment (198.3) | WB, IHC, FC | |
| TAB-030WM-F(E) | Anti-Human FAT1 Recombinant Antibody Fab Fragment (198.3) | WB, IHC, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0124-WJ-S(P) | Mouse Anti-FAT1 Recombinant Antibody; scFv Fragment (HPAB-0124-WJ-S(P)) | IHC, ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0124-WJ-F(E) | Mouse Anti-FAT1 Recombinant Antibody; Fab Fragment (HPAB-0124-WJ-F(E)) | IHC, ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC347 | Recombinant Anti-FAT1 Vesicular Antibody, EV Displayed (VS-0425-YC347) | ELISA, FC, Cell-uptake |
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