Rabbit Anti-FKBP4 Recombinant Antibody (clone R08-4F8)
CAT#: VS3-FY2004
The R08-4F8 recombinant monoclonal antibody detects human FKBP4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The binding specificity of the FKBP52 antibody was determined using western blot analysis of FKBP52 in Raw264.7 lysates and western blot analysis of FKBP52 in U2OS, C6 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6148/35_G6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62857/1230_E5_2_selected.jpg
Normal Tissue
Figure 3 Vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62857/168946_B_1_2.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62857/168946_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62857/168946_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62857/168946_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62857/168946_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004478-FKBP4
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WB
Figure 1 Anti-FKBP4 Antibody (VS3-FY2004) in WB.
The FKBP52 expression in Raw264.7 lysates were detected using the anti-FKBP52 by western blot assay.
WB
Figure 2 Anti-FKBP4 Antibody (VS3-FY2004) in WB.
The FKBP52 expression in U2OS, C6 lysates were detected using the anti-FKBP52 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human FKBP52
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat FKBP4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4F8
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 52 kDa, while the observed molecular weight stands at 52 kDa.
- Related Disease
- Paralytic Lagophthalmos and Facial Nerve Disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- FK506 Binding Protein 4; FK506 Binding Protein 4, 59kDa; EC 5.2.1.8; Rotamase; FKBP51; FKBP52; FKBP59; HBI; Peptidyl-Prolyl Cis-Trans Isomerase FKBP4; T-Cell FK506-Binding Protein, 59kD; Peptidylprolyl Cis-Trans Isomerase; FK506-Binding Protein 4 (59kD); 51 KDa FK506-Binding Protein; 52 KDa FK506-Binding Protein
- Gene ID
- 2288
- UniProt ID
- Q02790
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Microtubule, Mitochondrion, Nucleus
- Post Translation Modifications
- Phosphorylation by CK2 results in loss of HSP90 binding activity. (Q02790-FKBP4_HUMAN)
Ubiquitination at Lys28, Lys76, Lys83, Lys88, Lys222, Lys244, Lys250, Lys354, Lys378, and Lys409 (NX_Q02790 [NX_Q02790-1])
Modification sites at PhosphoSitePlus (Q02790)
Glycosylation from GlyGen (Q02790) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_047284495.1; NP_002005.1
- Function
- May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "FKBP4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0584CT | Recombinant Mouse anti-Human FKBP4 Monoclonal antibody (EML311) | ICC, IF, IHC-P, WB | |
| MRO-0594-CN | Rabbit Anti-FKBP4 Recombinant Antibody (clone JG96-30) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK337 | Rabbit Anti-FKBP4 Recombinant Antibody (clone JB95-39) | WB, ELISA | Rabbit IgG |
| VS3-FY2709 | Rabbit Anti-FKBP4 Recombinant Antibody (VS3-FY2709) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-843CQ | Mouse Anti-FKBP4 Recombinant Antibody (clone KN382/EC1) | IHC-Fr, WB, IP, RIA, ICC, Block, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1310 | Hi-Affi™ Rabbit Anti-FKBP4 Recombinant Antibody (clone DS1310AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY844 | Anti-FKBP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2563 | Anti-Mouse FKBP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2562 | Anti-Human FKBP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2564 | Anti-Rat FKBP4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC402 | CytoStream™ Rabbit Anti-FKBP4 Recombinant Antibody (VS13-YC402) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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