Mouse Anti-Flag Tag Recombinant Antibody (clone 4G5C8) (CAT#: ZG-0288U)
Mouse anti-Flag Tag antibody (clone 4G5C8) displayed high specificity against the Flag Tag.
Specific Inquiry
WB
Figure 1 Mouse Anti-Flag Tag Antibody (ZG-0288U) in WB
Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng All lanes: Flag Tag antibody at 1: 1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 59.6, 36, 35, 42 kDa
Observed band size: 59.6, 36, 35, 42 kDa
IP
Figure 2 Mouse Anti-Flag Tag Antibody (ZG-0288U) in IP
Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate. Lane 1: Mouse control IgG in Transfected HEK-293 cell whole cell lysate
Lane 2: ZG-0288U (1µl) + Transfected HEK-293 cell whole cell lysate (500µg)
Lane 3: Transfected HEK-293 cell whole cell lysate (20µg)
For western blotting, the blot was detected with ZG-0288U at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
IF
Figure 3 Mouse Anti-Flag Tag Antibody (ZG-0288U) in IF
Immunofluorescence staining of 293T cells transfected with Flag tag by ZG-0288U at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.
Specifications
- Immunogen
- DYKDDDDK(Flag) synthetic peptide conjugate to KLH
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Flag Tag
- Clone
- 4G5C8
- Applications
- ELISA, WB, IF, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl
Target
- Alternative Names
- DDK tag; DYKDDDDK; Flag; Flag Tag
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0062YJ | Human Anti-FLAG Tag Recombinant Antibody (clone CBS049) | ELISA | Human IgG |
| MRO-0221YJ | Rabbit Anti-Flag tag Recombinant Antibody (clone CBS170) | WB, IF, ICC, IP, ELISA | Rabbit IgG |
| VS3-WK1249 | Mouse Anti-Flag Tag Recombinant Antibody (VS3-WK1249) | ELISA, WB | Mouse IgG2b |
| VS3-WK1398 | Mouse Anti-Flag Tag Recombinant Antibody (clone 2C5) | WB | Mouse IgG1 |
| VS3-FY554 | Recombinant Rabbit Anti-Flag Tag Antibody (clone R06-1K3) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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