CytoStream™ Rabbit Anti-FLNA Recombinant Antibody (VS13-YC404)
CAT#: VS13-YC404
The CytoStream™ Rabbit Anti-FLNA Recombinant Antibody is specific for human and mouse FLNA. FLNA encodes an actin-binding protein facilitating cytoskeletal remodeling, impacting cell migration pathways; defects cause syndromes like PVNH and various dysplasias due to its structural network roles. The anti-FLNA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/368/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2925/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/356/if_selected.jpg
Normal Tissue
Figure 4 Seminal vesicle
High expression in seminal vesicle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1115/2818_A_8_6_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/356/2854_B_7_5.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/356/2854_A_8_4.jpg
Normal Tissue
Figure 7 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/356/2854_A_3_3.jpg
Normal Tissue
Figure 8 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/356/2854_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/356/2854_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/356/2854_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196924-FLNA
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Specifications
- Immunogen
- Recombinant human Filamin A, aa 2598-2647.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse FLNA
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/100-1/400
Flow cytometry (FC): 1/50
Target
- Alternative Names
- Filamin A; Endothelial Actin-Binding Protein; Actin Binding Protein 280; Non-Muscle Filamin; Filamin A, Alpha; Alpha-Filamin; Filamin-1; ABP-280; FLN-A; FLN1; FLN; Filamin A, Alpha (Actin Binding Protein 280); Actin-Binding Protein 280; Alpha Filamin; EC 2.1.1.43;
- Gene ID
- 2316
- UniProt ID
- P21333
- Long Name
- Filamin A
- Cellular Localization
- Cytoplasm
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "FLNA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2360CT | Recombinant Mouse anti-Human FLNA Monoclonal antibody (FQS4423) | IHC-P | |
| MRO-1867-CN | Rabbit Anti-FLNA Polyclonal Antibody (MRO-1867-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0670J | Rabbit Anti-FLNA Recombinant Antibody (clone 1B2) | ELISA, IHC | Rabbit IgG |
| VS3-QX432 | Mouse Anti-FLNA Recombinant Antibody (clone 1273CT424.104.153) | WB | Mouse IgG1 |
| VS3-CJ776 | Rabbit Anti-FLNA Recombinant Antibody (VS3-CJ776) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1318 | Hi-Affi™ Rabbit Anti-FLNA Recombinant Antibody (clone DS1318AB) | FC, ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY847 | Anti-FLNA Immunohistochemistry Kit | IHC | |
| VS-0525-XY2574 | Anti-Mouse FLNA Immunohistochemistry Kit | IHC | |
| VS-0525-XY2573 | Anti-Human FLNA Immunohistochemistry Kit | IHC | |
| VS-0525-XY2575 | Anti-Rat FLNA Immunohistochemistry Kit | IHC |
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