Human Anti-GalNAc Recombinant Antibody (HPAB-0447-FY)
CAT#: HPAB-0447-FY
This product is a recombinant human antibody that recognizes GalNAc. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Tested Data
SDS-PAGE
Figure 1 Human Anti-GalNAc Recombinant Antibody (HPAB-0447-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-0447-FY in non-reduced and β-mercaptoethanol-reduced conditions.
SEC-HPLC
Figure 2 Human Anti-GalNAc Recombinant Antibody (HPAB-0447-FY) in HPLC
The purity of HPAB-0447-FY was greater than 92% as determined by SEC-HPLC.
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human GalNAc
- Species Reactivity
- Human
- Applications
- IHC, ELISA
- Related Disease
- Inflammatory bowel disease (IBD)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- N-acetylglucosamine or N-acetyl-galactosamine; GalNAc
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Accurate Detection Of GalNAc
We needed a reliable antibody to detect N-Acetylgalactosamine residues in our tissue samples using Immunohistochemistry. This recombinant human antibody provided excellent staining patterns that matched our expected distribution for the amino sugar derivative. The defined culture media expression system seems to contribute to the high purity and lack of contaminating factors in the reagent.
Consistent Results In ELISA
Our lab used this antibody for ELISA assays to quantify GalNAc levels in various biological samples. The product demonstrated high affinity and consistent binding across multiple plates. Since it is a recombinant antibody, we have observed very little batch-to-batch variation, which is essential for the long-term tracking of our experimental data.
Q&As
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How is this antibody purified?
A: This recombinant human antibody is purified using affinity chromatography. It is expressed in mammalian cells using chemically defined culture media, which helps ensure a high level of purity (greater than 95% by SDS-PAGE) and consistency suitable for applications like IHC and ELISA.
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What buffer is this antibody supplied in?
A: This product is supplied in a PBS buffer. It does not contain any preservatives. Therefore, it is important to centrifuge the vial briefly before opening and to store aliquots at -20°C for the long term to maintain the stability and sterility of the antibody solution.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GalNAc"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-479 | Mouse Anti-GalNAc Recombinant Antibody (clone 237) | ELISA, WB, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-476 | Mouse Anti-GalNAc Recombinant Antibody (clone 237); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-476 | Mouse Anti-GalNAc Recombinant Antibody (clone 237); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0444-FY | Mouse Anti-GalNAc Recombinant Antibody (HPAB-0444-FY) | Inhib, ELISA, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0445-FY | Mouse Anti-GalNAc Recombinant Antibody (HPAB-0445-FY) | IF, ELISA, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0446-FY | Mouse Anti-GalNAc Recombinant Antibody (HPAB-0446-FY) | Inhib, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0444-FY-S(P) | Mouse Anti-GalNAc Recombinant Antibody; scFv Fragment (HPAB-0444-FY-S(P)) | Inhib, ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0445-FY-S(P) | Mouse Anti-GalNAc Recombinant Antibody; scFv Fragment (HPAB-0445-FY-S(P)) | IF, ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0446-FY-S(P) | Mouse Anti-GalNAc Recombinant Antibody; scFv Fragment (HPAB-0446-FY-S(P)) | Inhib, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0447-FY-S(P) | Human Anti-GalNAc Recombinant Antibody; scFv Fragment (HPAB-0447-FY-S(P)) | IHC, ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0444-FY-F(E) | Mouse Anti-GalNAc Recombinant Antibody; Fab Fragment (HPAB-0444-FY-F(E)) | Inhib, ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0445-FY-F(E) | Mouse Anti-GalNAc Recombinant Antibody; Fab Fragment (HPAB-0445-FY-F(E)) | IF, ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0446-FY-F(E) | Mouse Anti-GalNAc Recombinant Antibody; Fab Fragment (HPAB-0446-FY-F(E)) | Inhib, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0447-FY-F(E) | Human Anti-GalNAc Recombinant Antibody; Fab Fragment (HPAB-0447-FY-F(E)) | IHC, ELISA | Humanized Fab |
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