Mouse Anti-GFP Recombinant Antibody (ZG-0278U) (CAT#: ZG-0278U)

Mouse anti-GFP antibody displayed high specificity against the GFP.

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WB

Figure 1 Mouse Anti-GFP Antibody (ZG-0278U) in WB

Figure 1 Mouse Anti-GFP Antibody (ZG-0278U) in WB

Mouse Anti-GFP monoclonal antibody at 0.1μg/ml Line 1: HEK-293 cell lysate
Line 2: GFP transfected HEK-293 cell lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd

IP

Figure 2 Mouse Anti-GFP Antibody (ZG-0278U) in IP

Figure 2 Mouse Anti-GFP Antibody (ZG-0278U) in IP

Result of anti-GFP-tag Monoclonal antibody Line 1:Control IgG
Line 2:Precipitateing GFP-tagged fusion protein
Line 3:GFP Transfected HEK-293 cells lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd


Specifications

  • Immunogen
  • Recombinant GFP Protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b
  • Specificity
  • GFP
  • Species Reactivity
  • All
  • Applications
  • ELISA, WB, IP
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IP:1:200-1:2000

Target

  • Alternative Names
  • CFP; eGFP; eYFP; GFP; GFP tag; YFP

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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