Rabbit Anti-GFPT2 Recombinant Antibody (clone R09-7H8)
CAT#: VS3-FY1728
The R09-7H8 recombinant monoclonal antibody specifically recognizes human GFPT2. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. The antibody has the positive detection of GFPT2 protein by western blot analysis of GFPT2 in 3T3, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-GFPT2 Antibody (VS3-FY1728) in WB.
The GFPT2 expression in 3T3, Hela lysates were detected using the anti-GFPT2 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56892/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/1125_G12_2_blue_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/1089_C9_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/133565_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/133565_A_8_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/133565_A_7_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/133565_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/133565_A_5_6.jpg
Normal Tissue
Figure 11 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59910/133565_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131459-GFPT2
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Specifications
- Immunogen
- Recombinant human GFPT2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GFPT2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-7H8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 77 kDa.
- Related Disease
- Type 2 Diabetes Mellitus and Diabetes Mellitus
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- GFAT; GFAT2; GFAT 2
- Gene ID
- 9945
- UniProt ID
- O94808
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_005101.1
- Function
- Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GFPT2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1430 | Hi-Affi™ Rabbit Anti-GFPT2 Recombinant Antibody (clone DS1430AB) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC446 | Rabbit Anti-GFPT2 Recombinant Antibody (VS13-YC446) | WB, ICC, IF, IP | Rabbit IgG |
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