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  • Rabbit Anti-GOLGA2 Recombinant Antibody (clone R07-3D2)

Rabbit Anti-GOLGA2 Recombinant Antibody (clone R07-3D2) (CAT#: VS3-XY3369)

This rabbit recombinant antibody specifically recognizes GOLGA2 (Golgin A2), which plays a key role in spindle pole assembly and centrosome organization. It reacts with Human, Mouse, Rat, Monkey, Cow and Dog GOLGA2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.


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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21178/189_B5_1_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15421/33797_A_9_2_selected.jpg
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_B_7_5.jpg
Figure 5 Colon
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_8_3.jpg
Figure 6 Pancreas
Figure 6 Pancreas
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_3_3.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_8_5.jpg
Figure 8 Testis
Figure 8 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167110-GOLGA2

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse Rat, Monkey, Cow and Dog GOLGA2
  • Species Reactivity
  • Human, Mouse, Rat, Monkey, Cow, Dog
  • Clone
  • R07-3D2
  • Applications
  • WB, IHC-P, ICC, IF, IP
  • Related Disease
  • Neuromuscular Disease

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IHC: 1/50-1/100
    IF: 1/50-1/200
    IP: 1/20

Target

  • Alternative Names
  • GM130; DEDHMB
  • Long Name
  • Golgin A2
  • Cellular Localization
  • Cytoplasm, cytoskeleton, spindle pole, Golgi apparatus
  • Post Translation Modifications
  • Modification sites at PhosphoSitePlus (Q08379)
    Modification sites at neXtProt (NX_Q08379)
    Glycosylation from GlyGen (Q08379) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity).
    Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940).
    Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "GOLGA2"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1473 Hi-Affi™ Rabbit Anti-GOLGA2 Recombinant Antibody (clone DS1473AB) FC, WB Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
MRO-1899-CN Rabbit Anti-GOLGA2 Polyclonal Antibody (MRO-1899-CN) WB, IF, IHC, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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