Rabbit Anti-GOLGA2 Recombinant Antibody (clone R07-3D2)
CAT#: VS3-XY3369
This rabbit recombinant antibody specifically recognizes GOLGA2 (Golgin A2), which plays a key role in spindle pole assembly and centrosome organization. It reacts with Human, Mouse, Rat, Monkey, Cow and Dog GOLGA2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21178/189_B5_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15421/33797_A_9_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/if_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_8_3.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21799/49330_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167110-GOLGA2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse Rat, Monkey, Cow and Dog GOLGA2
- Species Reactivity
- Human, Mouse, Rat, Monkey, Cow, Dog
- Clone
- R07-3D2
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Neuromuscular Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- GM130; DEDHMB
- Gene ID
- 2801
- UniProt ID
- Q08379
- Long Name
- Golgin A2
- Cellular Localization
- Cytoplasm, cytoskeleton, spindle pole, Golgi apparatus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q08379)
Modification sites at neXtProt (NX_Q08379)
Glycosylation from GlyGen (Q08379) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001353173.2
- Function
- Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity).
Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940).
Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GOLGA2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1473 | Hi-Affi™ Rabbit Anti-GOLGA2 Recombinant Antibody (clone DS1473AB) | FC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1899-CN | Rabbit Anti-GOLGA2 Polyclonal Antibody (MRO-1899-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS7-HM1014 | Mouse Anti-GOLGA2 Recombinant Antibody (clone 2D3D12) | WB, IHC, ICC, FCM | Mouse IgG1 |
| VS7-HM1015 | Mouse Anti-GOLGA2 Recombinant Antibody (clone 2D5D11) | WB, IHC, ICC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY937 | Anti-GOLGA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2858 | Anti-Rat GOLGA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2859 | Anti-Mouse GOLGA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2857 | Anti-Human GOLGA2 Immunohistochemistry Kit | IHC |
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