Rabbit Anti-GRK2 Polyclonal Antibody (CAT#: VS3-WK1568)
This antibody is a rabbit polyclonal antibody against human GRK2, which can phosphorylate the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, cytoskeletal, mitochondrial, and transcription factor proteins. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen (synthesized peptide derived from human GRK2). It can detect endogenous levels of GRK2. This product was tested for use in WB and ELISA.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60707/1779_F5_2_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60707/1779_F5_2_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_8_8.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_8_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_9_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_9_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37248/86033_A_5_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (NB-4, REH)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173020-GRK2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173020-GRK2
Specifications
- Immunogen
- Synthesized peptide derived from human GRK2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GRK2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500) and ELISA (1:10000-1/20000).
Target
- Alternative Names
- G Protein-Coupled Receptor Kinase 2; EC 2.7.11.15; Beta-ARK-1; ADRBK1; BARK1; Adrenergic, Beta, Receptor Kinase 1; G-Protein Coupled Receptor Kinase 2
- Gene ID
- 156
- UniProt ID
- P25098
- Long Name
- G Protein-Coupled Receptor Kinase 2
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse
- Protein Refseq
- NP_001610.2
- Function
- Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Hedgehog Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GRK2"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0093 | Rabbit Anti-GRK2 Recombinant Antibody (clone DS93AB) | WB, IHC-P, ICC, FC, IP | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4419 | PE-B*07:02/Human GRK2 (KPRSPVVEL) MHC Tetramer | FCM |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0694-CN | Rabbit Anti-GRK2 Recombinant Antibody (clone CBACN-250) | WB, IHC | Rabbit IgG |
| ZG-0395J | Mouse Anti-GRK2 Recombinant Antibody (ZG-0395J) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0396J | Mouse Anti-GRK2 Recombinant Antibody (ZG-0396J) | WB | Mouse IgG |
| VS3-QX478 | Mouse Anti-GRK2 Recombinant Antibody (clone 6C1-D9-C0) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ817 | Rabbit Anti-GRK2 Recombinant Antibody (VS3-CJ817) | WB, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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