Rabbit Anti-GRSF1 Recombinant Antibody (clone R06-2B5)
CAT#: VS3-FY2008
The anti-GRSF1 antibody plays a role in western blot analysis of GRSF1 in Hela, Ramos lysates. This is a rabbit recombinant monoclonal antibody with a high affinity of human GRSF1. It is suitable for use in a variety of assays, including WB, ICC/IF.
Tested Data
WB
Figure 1 Anti-GRSF1 Antibody (VS3-FY2008) in WB.
The GRSF1 expression in Hela, Ramos lysates were detected using the anti-GRSF1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human GRSF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GRSF1
- Species Reactivity
- Human
- Clone
- R06-2B5
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.
- Related Disease
- Ovarian Melanoma and Spastic Paraplegia 7, Autosomal Recessive
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- G-Rich RNA Sequence Binding Factor 1; GRSF-1; G-Rich Sequence Factor 1
- Gene ID
- 2926
- UniProt ID
- Q12849
- Cellular Localization
- Cytoplasm, Mitochondrion
- Post Translation Modifications
- Ubiquitination at Lys242 (NX_Q12849 [NX_Q12849-1])
Modification sites at PhosphoSitePlus (Q12849)
Glycosylation from GlyGen (Q12849) 4 sites, 2 O-linked glycans (4 sites)
- Protein Refseq
- XP_011530199.1; XP_047306096.1; XP_054205813.1; XP_054205814.1; NP_001091947.1
- Function
- Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome.
G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GRSF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC475 | Rabbit Anti-GRSF1 Recombinant Antibody (VS13-YC475) | WB, ICC, IF | Rabbit IgG |
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