Rabbit Anti-H2AC4 Recombinant Antibody (clone 1H12) (CAT#: ZG-0717J)
This product is a rabbit antibody that recognizes H2AC4.
Specific Inquiry
WB
Figure 1 Rabbit Anti-H2AC4 Antibody (ZG-0717J) in WB
Western Blot
Positive WB detected in HepG2 whole cell lysate
All lanes Histone H2A type 1-B/E antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 KDa
Observed band size: 13 KDa
IF
Figure 2 Rabbit Anti-H2AC4 Antibody (ZG-0717J) in IF
Immunofluorescence staining of Hela cells with this product at 1:168,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human H2AC4
- Species Reactivity
- Human
- Clone
- 1H12
- Applications
- ELISA, WB, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
WB:1:500-1:5000, IF:1:30-1:200
Target
- Alternative Names
- H2A/m; H2AC8; H2AFM; HIST1H2AB; histone H2A type 1-B/E; H2A histone family, member M; histone 1, H2ab; histone H2A/m; histone cluster 1 H2A family member b; histone cluster 1, H2ab
- Gene ID
- 8335
- UniProt ID
- P04908
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "H2AC4"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0715J | Rabbit Anti-H2AC4 Recombinant Antibody (clone 3H5) | ELISA, ICC, IF | Rabbit IgG |
| ZG-0716J | Rabbit Anti-H2AC4 Recombinant Antibody (clone 3B12) | ELISA, WB | Rabbit IgG |
| VS3-CJ384 | Rabbit Anti-H2AC4 Recombinant Antibody (VS3-CJ384) | WB, ICC, IF, IHC | Rabbit IgG |
| VS3-CJ385 | Rabbit Anti-H2AC4 Recombinant Antibody (VS3-CJ385) | WB, IHC | Rabbit IgG |
| VS3-CJ386 | Rabbit Anti-H2AC4 Recombinant Antibody (VS3-CJ386) | WB, ICC, IF, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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