Rabbit Anti-H2AFY Recombinant Antibody (VS13-YC493) (CAT#: VS13-YC493)
The Rabbit Anti-H2AFY Recombinant Antibody is specific for human, mouse and rat H2AFY. H2AFY relates to a replication-independent histone part of the H2A family, replacing conventional H2A histones in certain nucleosomes. It represses transcription and participates in stable X chromosome inactivation, with multiple transcript variants resulting from alternative splicing. The anti-H2AFY antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50962/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50962/if_selected.jpg
Figure 3 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/ihc_selected.jpg
Figure 4 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_B_7_5.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_B_7_5.jpg
Figure 5 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_7_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_7_3.jpg
Figure 6 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_8_4.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_7_5.jpg
Figure 8 Testis
(Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_6_6.jpg
(Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_6_6.jpg
Figure 9 Skin
(Fibroblasts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Langerhans
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Melanocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_B_7_1.jpg
(Fibroblasts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Langerhans
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Melanocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_B_7_1.jpg
Figure 10 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/102310_A_9_8.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113648-MACROH2A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113648-MACROH2A1
Specifications
- Immunogen
- Recombinant human macroH2A.1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat H2AFY
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- H2A Histone Family Member Y; Medulloblastoma Antigen MU-MB-50.205; Histone MacroH2A1; Histone H2A.Y; H2A/Y; MH2A1; H2A Histone Family, Member Y; Core Histone Macro-H2A.1;
- Gene ID
- 9555
- UniProt ID
- O75367
- Long Name
- H2A Histone Family Member Y
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "H2AFY"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1412z | Mouse Anti-H2AFY Recombinant Antibody (clone 33G8) | WB, IHC | Mouse IgG1 |
| MOB-1106CT | Recombinant Mouse anti-Human H2AFY Monoclonal antibody (25H8) | ELISA, WB | |
| MRO-0953-CN | Rabbit Anti-H2AFY Recombinant Antibody (clone JU22-83) | WB, IF, IHC | Rabbit IgG |
| MRO-2022-CN | Rabbit Anti-H2AFY Polyclonal Antibody (MRO-2022-CN) | WB, IHC, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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