Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) (CAT#: MOB-0020F)
This product is a mouse antibody that recognizes H3F3A.
Specific Inquiry
IF
Figure 1 Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) (MOB-0020F) in IF
Immunofluorescence analysis of Hela cells. 1. Amyloid β polyclonal antibody (green) is diluted at 1:200 (overnight at 4°C). (Red) Dilute at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 488 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor594 diluted 1:1000 (room temperature, 50 minutes).
WB
Figure 2 Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) (MOB-0020F) in WB
Western blot analysis of lysates from 1) Hela, 2) Raw, 3) mouse brain tissue cells, 4) rat brain tissue cells, (green) primary antibody diluted 1:1000, 4°C overnight, Dylight 800 Secondary antibody (diluted to 1:10000, 37°C for 1 hour. (red) Actin β polyclonal antibody diluted 1:5000 as loading control, overnight at 4°C, Dylight 680 secondary antibody (diluted 1:10000, 37°C) 1 hour.
IHC
Figure 3 Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) (MOB-0020F) in IHC
Immunohistochemical analysis of paraffin-embedded human uterine tissue. 1. Histone H3 Monoclonal Antibody (1G1) 1:200 dilution (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human H3F3A
- Species Reactivity
- Human, Mouse, Rat, Yeast
- Clone
- 1G1
- Applications
- WB, IHC-P, IF, IP
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistologyp-Paraffin Sections, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; HistoneH3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3 Histone Family Member 3A; H3 Histone, Family 3A; H3.3A; H3F3; Histone H3.3; H3F3A H3F3B; H3.3B
- Gene ID
- 3020
- UniProt ID
- P84243
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1556 | Rabbit Anti-H3F3A Recombinant Antibody (clone DS1556AB) | WB, ChIP, DB, PIA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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