Mouse Anti-H3F3A Recombinant Antibody (clone 1G1)
CAT#: MOB-0020F
This product is a mouse antibody that recognizes H3F3A.
Tested Data
Gene Expression
IF
Figure 1 Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) (MOB-0020F) in IF
Immunofluorescence analysis of Hela cells. 1. Amyloid β polyclonal antibody (green) is diluted at 1:200 (overnight at 4°C). (Red) Dilute at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 488 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor594 diluted 1:1000 (room temperature, 50 minutes).
WB
Figure 2 Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) (MOB-0020F) in WB
Western blot analysis of lysates from 1) Hela, 2) Raw, 3) mouse brain tissue cells, 4) rat brain tissue cells, (green) primary antibody diluted 1:1000, 4°C overnight, Dylight 800 Secondary antibody (diluted to 1:10000, 37°C for 1 hour. (red) Actin β polyclonal antibody diluted 1:5000 as loading control, overnight at 4°C, Dylight 680 secondary antibody (diluted 1:10000, 37°C) 1 hour.
IHC
Figure 3 Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) (MOB-0020F) in IHC
Immunohistochemical analysis of paraffin-embedded human uterine tissue. 1. Histone H3 Monoclonal Antibody (1G1) 1:200 dilution (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11481/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37178/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11481/32905_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11481/32905_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11481/32905_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11481/32905_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11481/32905_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11481/32905_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163041-H3F3A
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- Human H3F3A
- Species Reactivity
- Human, Mouse, Rat, Yeast
- Clone
- 1G1
- Applications
- WB, IHC-P, IF, IP
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistologyp-Paraffin Sections, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; HistoneH3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3 Histone Family Member 3A; H3 Histone, Family 3A; H3.3A; H3F3; Histone H3.3; H3F3A H3F3B; H3.3B
- Gene ID
- 3020
- UniProt ID
- P84243
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for MOB-0020F. Click the button above to contact us or submit your feedback about this product.
Strong Nuclear Signal in IHC
We used this mouse antibody (clone 1G1) for Immunohistochemistry on paraffin sections to detect H3F3A. The nuclear staining was strong and specific, highlighting the chromatin structure as expected for a histone protein. It worked well in both human and mouse tissue samples, showing its broad cross-reactivity.
Excellent Western Blot Performance
This antibody is great for Western Blotting of histone extracts. It specifically recognized H3F3A (Histone H3.3) without excessive background. We appreciate that it can also be used for Immunoprecipitation, allowing us to isolate nucleosomes containing this specific variant for further downstream sequencing analysis in our epigenetics research.
Q&As
-
Which species does this H3F3A antibody react with?
A: This Mouse Anti-H3F3A Recombinant Antibody (clone 1G1) reacts with Human, Mouse, Rat, and Yeast. It targets H3F3A (Histone H3.3), a replication-independent member of the histone H3 family. Its broad reactivity makes it suitable for comparative studies across different organisms.
-
Can I use this antibody for paraffin-embedded tissue sections?
A: Yes, this antibody has been tested for use in Immunohistology-Paraffin Sections (IHC-P). It is also suitable for Western Blot (WB), Immunofluorescence (IF), and Immunoprecipitation (IP). It helps visualize histones which are responsible for the nucleosome structure of the chromosomal fiber.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "H3F3A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1556 | Rabbit Anti-H3F3A Recombinant Antibody (clone DS1556AB) | WB, ChIP, DB, PIA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2999 | PE-Mamu-B*003:01/Human H3F3A (RRYQKSTEL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0749-CN | Rabbit Anti-H3F3A Recombinant Antibody (clone CBACN-275) | WB, IF, IHC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2405-CN | Rabbit Anti-H3F3A Recombinant Antibody (clone CBACN-688) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0001F | Mouse Anti-H3F3A Recombinant Antibody (clone 1A8) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0002F | Mouse Anti-H3F3A Recombinant Antibody (clone 3B12) | WB, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0003F | Mouse Anti-H3F3A Recombinant Antibody (clone 1E6) | WB | Mouse IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
