Rabbit Anti-H4C14 Recombinant Antibody (clone 2C9) (CAT#: ZG-0735J)

Figure 1 Rabbit Anti-H4C14 Antibody (ZG-0735J) in WB

Western Blot
Positive WB detected in Hela whole cell lysate,MCF-7 whole cell lysate,293 whole cell lysate,HepG2 whole cell lysate
All lanes Acetyl-Histone H4 (K5) antibody at 1.05μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 KDa
Observed band size: 11 KDa

Figure 2 Rabbit Anti-H4C14 Antibody (ZG-0735J) in ICC

Immunocytochemistry analysis of this product diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-H4C14 Antibody (ZG-0735J) in IF

Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with this product at 1:65,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).