Recombinant Human Anti-H5N1 Antibody (AV.G4) (CAT#: HPAB-AP660-YC)

Figure 1 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-AP660-YC in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in SEC-HPLC

The purity of HPAB-AP660-YC was 95.4445% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µlInjection: 10 µl

Figure 3 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in WB

Western blot analysis of HPAB-AP660-YC was performed by loading Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-AP660-YC and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 4 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in DB

Dot Blot analysis of HPAB-AP660-YC was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag).

HPAB-AP660-YC incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)

Figure 5 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in ELISA

ELISA analysis of HPAB-AP660-YC was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag). Then blocked with BSA and incubated with HPAB-AP660-YC. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.