Recombinant Human Anti-H5N1 Antibody (AV.G4) (CAT#: HPAB-AP660-YC)
Provided are antibodies useful in recognising multiple clades of H5N1 avian influenza virus. The antibody can be used for immunoassay techniques such as: ELISA and Western Blot.
Specific Inquiry
SDS-PAGE
Figure 1 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in SDS-PAGE
SDS-PAGE analysis of HPAB-AP660-YC in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in SEC-HPLC
The purity of HPAB-AP660-YC was 95.4445% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µlInjection: 10 µl
WB
Figure 3 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in WB
Western blot analysis of HPAB-AP660-YC was performed by loading Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-AP660-YC and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
DB
Figure 4 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in DB
Dot Blot analysis of HPAB-AP660-YC was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag).
HPAB-AP660-YC incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in ELISA
ELISA analysis of HPAB-AP660-YC was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag). Then blocked with BSA and incubated with HPAB-AP660-YC. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- H5N1
- Species Reactivity
- H5N1
- Clone
- AV.G4
- Applications
- ELISA, Western Blot
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Target
- Alternative Names
- Influenza virus H5N1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone AV.G4"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-427 | Recombinant Anti-IAV H5N1 Antibody | ELISA, WB, Dot, FuncS | IgG |
| FAMAB-0238-CN | Recombinant Macaque Anti-H5N1 Antibody (32ku) | WB, ELISA, IF | Macaque IgG |
| HPAB-1166-FY | Human Anti-H5N1 Recombinant Antibody (HPAB-1166-FY) | ELISA, WB | Human IgG |
| HPAB-1167-FY | Human Anti-H5N1 Recombinant Antibody (HPAB-1167-FY) | ELISA, Neut | Human IgG |
| HPAB-1168-FY | Human Anti-H5N1 Recombinant Antibody (HPAB-1168-FY) | ELISA, Neut | Human IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-427-S(P) | Recombinant Anti-IAV H5N1 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| FAMAB-0238-CN-S(P) | Recombinant Macaque Anti-H5N1 Antibody scFv Fragment (32ku) | WB, ELISA, IF | Macaque scFv |
| HPAB-1166-FY-F(E) | Human Anti-H5N1 Recombinant Antibody; Fab Fragment (HPAB-1166-FY-F(E)) | ELISA, Neut | Human Fab |
| HPAB-1167-FY-F(E) | Human Anti-H5N1 Recombinant Antibody; Fab Fragment (HPAB-1167-FY-F(E)) | ELISA, Neut | Human Fab |
| HPAB-1168-FY-F(E) | Human Anti-H5N1 Recombinant Antibody; Fab Fragment (HPAB-1168-FY-F(E)) | ELISA, Neut | Human Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-076 | Recombinant Anti-H5N1 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0238-CN-F(E) | Recombinant Macaque Anti-H5N1 Antibody Fab Fragment (32ku) | WB, ELISA, IF | Macaque Fab |
| HPAB-1166-FY-S(P) | Human Anti-H5N1 Recombinant Antibody; scFv Fragment (HPAB-1166-FY-S(P)) | ELISA, Neut | Human scFv |
| HPAB-1167-FY-S(P) | Human Anti-H5N1 Recombinant Antibody; scFv Fragment (HPAB-1167-FY-S(P)) | ELISA, Neut | Human scFv |
| HPAB-1168-FY-S(P) | Human Anti-H5N1 Recombinant Antibody; scFv Fragment (HPAB-1168-FY-S(P)) | ELISA, Neut | Human scFv |
| HPAB-AP660-YC--F(E) | Recombinant Human Anti-H5N1 Antibody Fab Fragment (AV.G4) | ELISA, WB | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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