Recombinant Human Anti-H5N1 Antibody (AV.G4)
CAT#: HPAB-AP660-YC
Provided are antibodies useful in recognising multiple clades of H5N1 avian influenza virus. The antibody can be used for immunoassay techniques such as: ELISA and Western Blot.
Tested Data
SDS-PAGE
Figure 1 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in SDS-PAGE
SDS-PAGE analysis of HPAB-AP660-YC in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in SEC-HPLC
The purity of HPAB-AP660-YC was 95.4445% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µlInjection: 10 µl
WB
Figure 3 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in WB
Western blot analysis of HPAB-AP660-YC was performed by loading Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-AP660-YC and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
DB
Figure 4 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in DB
Dot Blot analysis of HPAB-AP660-YC was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag).
HPAB-AP660-YC incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Recombinant Human Anti-H5N1 Antibody (HPAB-AP660-YC) in ELISA
ELISA analysis of HPAB-AP660-YC was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) protein (His tag). Then blocked with BSA and incubated with HPAB-AP660-YC. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- H5N1
- Species Reactivity
- H5N1
- Clone
- AV.G4
- Applications
- ELISA, Western Blot
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Target
- Alternative Names
- Influenza virus H5N1
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for HPAB-AP660-YC. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone AV.G4"
- CAT
- Product Name
See other products for "H5N1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-427 | Recombinant Anti-IAV H5N1 Antibody | ELISA, WB, Dot, FuncS | IgG |
| FAMAB-0238-CN | Recombinant Macaque Anti-H5N1 Antibody (32ku) | WB, ELISA, IF | Macaque IgG |
| HPAB-1166-FY | Human Anti-H5N1 Recombinant Antibody (HPAB-1166-FY) | ELISA, WB | Human IgG |
| HPAB-1167-FY | Human Anti-H5N1 Recombinant Antibody (HPAB-1167-FY) | ELISA, Neut | Human IgG |
| HPAB-1168-FY | Human Anti-H5N1 Recombinant Antibody (HPAB-1168-FY) | ELISA, Neut | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-427-S(P) | Recombinant Anti-IAV H5N1 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| FAMAB-0238-CN-S(P) | Recombinant Macaque Anti-H5N1 Antibody scFv Fragment (32ku) | WB, ELISA, IF | Macaque scFv |
| HPAB-1166-FY-F(E) | Human Anti-H5N1 Recombinant Antibody; Fab Fragment (HPAB-1166-FY-F(E)) | ELISA, Neut | Human Fab |
| HPAB-1167-FY-F(E) | Human Anti-H5N1 Recombinant Antibody; Fab Fragment (HPAB-1167-FY-F(E)) | ELISA, Neut | Human Fab |
| HPAB-1168-FY-F(E) | Human Anti-H5N1 Recombinant Antibody; Fab Fragment (HPAB-1168-FY-F(E)) | ELISA, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-076 | Recombinant Anti-H5N1 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0238-CN-F(E) | Recombinant Macaque Anti-H5N1 Antibody Fab Fragment (32ku) | WB, ELISA, IF | Macaque Fab |
| HPAB-1166-FY-S(P) | Human Anti-H5N1 Recombinant Antibody; scFv Fragment (HPAB-1166-FY-S(P)) | ELISA, Neut | Human scFv |
| HPAB-1167-FY-S(P) | Human Anti-H5N1 Recombinant Antibody; scFv Fragment (HPAB-1167-FY-S(P)) | ELISA, Neut | Human scFv |
| HPAB-1168-FY-S(P) | Human Anti-H5N1 Recombinant Antibody; scFv Fragment (HPAB-1168-FY-S(P)) | ELISA, Neut | Human scFv |
| HPAB-AP660-YC--F(E) | Recombinant Human Anti-H5N1 Antibody Fab Fragment (AV.G4) | ELISA, WB | Human Fab |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
-2-1.png)
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: ELISA, FC
-3.jpg)
Application: Neut
Application: ELISA, FC
Application: ELISA, Block, WB, FC, IP
Application: ELISA, Neut, FuncS
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
