Rabbit Anti-HAT1 Recombinant Antibody (clone R04-3G4)
CAT#: VS3-FY1620
The R04-3G4 recombinant monoclonal antibody specifically binds to human HAT1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. Applications Tested: The KAT1 antibody has been tested by western blot analysis of KAT1/HAT1 in K562, C6, 3T3, Hela lysates and immunohistochemistry analysis of paraffin-embedded human tonsil.
Tested Data
WB
Figure 1 Anti-HAT1 Antibody (VS3-FY1620) in WB.
The KAT1/HAT1 expression in K562, C6, 3T3, Hela lysates were detected using the anti-KAT1/HAT1 by western blot assay.
IHC
Figure 2 Anti-HAT1 Antibody (VS3-FY1620) in IHC.
Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-KAT1/HAT1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Recombinant human KAT1/HAT1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HAT1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3G4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 50 kDa, while the observed molecular weight stands at 45 kDa.
- Related Disease
- Cheilitis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Histone Acetyltransferase 1; KAT1; Histone Acetyltransferase Type B Catalytic Subunit; EC 2.3.1.48
- Gene ID
- 8520
- UniProt ID
- O14929
- Sequence Similarities
- Belongs to the HAT1 family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Nucleus
- Post Translation Modifications
- Phosphorylated by AMPK at Ser-190; phosphorylation increases HAT1 activity.
- Protein Refseq
- NP_003633.2
- Function
- Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair.
Coordinates histone production and acetylation via H4 promoter binding.
Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac).
Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression.
Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks.
In addition, acetylates other substrates such as chromatin-related proteins.
Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HAT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ71 | Mouse Anti-HAT1 Recombinant Antibody (VS3-CJ71) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC683 | AbPlus™ Anti-HAT1 Magnetic Beads (VS-0724-YC683) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY993 | Anti-HAT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3058 | Anti-Mouse HAT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3057 | Anti-Human HAT1 Immunohistochemistry Kit | IHC |
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