Mouse Anti-HDAC4 Recombinant Antibody (ZG-0417J)
CAT#: ZG-0417J
This product is a mouse antibody that recognizes HDAC4.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-HDAC4 Recombinant Antibody (ZG-0417J) in WB
Western blot detection of HDAC4 in Jurkat,Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted) Predicted band size: 140KDa.Observed band size: 140KDa.
IP
Figure 2 Mouse Anti-HDAC4 Recombinant Antibody (ZG-0417J) in IP
Immunoprecipitation analysis of Hela cell lysates using HDAC4 mouse mAb.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm, nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48723/1855_B7_15_cr5ad8881abe486_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48723/736_E10_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_6_6.jpg
Normal Tissue
Figure 8 Skeletal muscle
Myocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_B_2_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000068024-HDAC4
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Specifications
- Immunogen
- Purified recombinant human HDAC4 protein fragments expressed in E.coli
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HDAC4
- Species Reactivity
- Human
- Applications
- WB
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot.
Target
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Related Diseases
Alcoholism
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "HDAC4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1576 | Rabbit Anti-HDAC4 Recombinant Antibody (clone DS1576AB) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0721-CN | Rabbit Anti-HDAC4 Recombinant Antibody (clone CBACN-260) | WB, IF | Rabbit IgG |
| ZG-0415J | Mouse Anti-HDAC4 Recombinant Antibody (ZG-0415J) | WB, ELISA | Mouse IgG |
| ZG-0416J | Mouse Anti-HDAC4 Recombinant Antibody (ZG-0416J) | WB | Mouse IgG |
| VS3-CJ1052 | Rabbit Anti-HDAC4 Recombinant Antibody (VS3-CJ1052) | WB, ICC, IF | Rabbit IgG |
| VS4-WK212 | Mouse Anti-HDAC4 Recombinant Antibody (VS4-WK212) | ELISA, WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1024 | AbPlus™ Anti-HDAC4 Magnetic Beads (VS-0724-YC1024) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1002 | Anti-HDAC4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3098 | Anti-Human HDAC4 Immunohistochemistry Kit | IHC |
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