Rabbit Anti-HDAC9 Recombinant Antibody (clone R03-3C3)
CAT#: VS3-XY3378
It is a recombinant rabbit antibody targeting the HDAC9 (Histone deacetylase 9), which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28926/if_selected.jpg
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat HDAC9
- Species Reactivity
- Human, Rat
- Clone
- R03-3C3
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Auriculocondylar Syndrome 4; Primary Cutaneous T-Cell Non-Hodgkin Lymphoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; ARCND4; HDAC7B; HDAC9B; HDAC9FL
- Gene ID
- 9734
- UniProt ID
- Q9UKV0
- Long Name
- Histone deacetylase 9
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity.
Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity).
Isoform 7 is phosphorylated on Tyr-1010.
Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
- Protein Refseq
- NP_001191073.1; NP_001191074.1
- Function
- Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4).
Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Represses MEF2-dependent transcription.
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Related Diseases
Alcoholism
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HDAC9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1580 | Rabbit Anti-HDAC9 Recombinant Antibody (clone DS1580AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0726-CN | Rabbit Anti-HDAC9 Recombinant Antibody (clone JU30-44) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-CJ11 | Mouse Anti-HDAC9 Recombinant Antibody (VS3-CJ11) | WB, ELISA | Mouse IgG |
| VS3-XY801 | Mouse Anti-HDAC9 Recombinant Antibody (clone B486A1) | ELISA, WB, FC | Mouse IgG1 |
| VS3-FY664 | Recombinant Rabbit Anti-HDAC9 Antibody (clone R01-8I6) | WB, IHC-P | Rabbit IgG |
| VS7-0425-WR29 | Mouse Anti-HDAC9 Recombinant Antibody (clone 2B7C4) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3103 | Anti-HDAC9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3104 | Anti-Mouse HDAC9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3105 | Anti-Rat HDAC9 Immunohistochemistry Kit | IHC |
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